Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX
Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is r...
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ftsmithonian:oai:figshare.com:article/17119886 2023-05-15T15:59:08+02:00 Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX Jiulin Chan (3276780) Lu Wang (45927) Li Li (14993) Kang Mu (11787512) David Bushek (11787515) Yue Xu (246925) Ximing Guo (66272) Guofan Zhang (126339) Linlin Zhang (131810) 2021-12-03T04:39:58Z https://doi.org/10.3389/fgene.2021.795706.s004 unknown https://figshare.com/articles/dataset/Table1_Transcriptomic_Response_to_Perkinsus_marinus_in_Two_Crassostrea_Oysters_Reveals_Evolutionary_Dynamics_of_Host-Parasite_Interactions_DOCX/17119886 doi:10.3389/fgene.2021.795706.s004 CC BY 4.0 CC-BY Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation Dataset 2021 ftsmithonian https://doi.org/10.3389/fgene.2021.795706.s004 2021-12-19T20:14:58Z Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is resistant to Dermo due to differences in the host-parasite interaction that is not well understood. We compared transcriptomic responses to P. marinus challenge in the two oysters at early and late infection stages. Dynamic and orchestrated regulation of large sets of innate immune response genes were observed in both species with remarkably similar patterns for most orthologs, although responses in C. virginica were stronger, suggesting strong or over-reacting immune response could be a cause of host mortality. Between the two species, several key immune response gene families differed in their expansion, sequence variation and/or transcriptional response to P. marinus, reflecting evolutionary divergence in host-parasite interaction. Of note, significant upregulation of inhibitors of apoptosis (IAPs) was observed in resistant C. gigas but not in susceptible C. virginica, suggesting upregulation of IAPs is an active defense mechanism, not a passive response orchestrated by P. marinus. Compared with C. gigas, C. virginica exhibited greater expansion of toll-like receptors (TLRs) and positive selection in P. marinus responsive TLRs. The C1q domain containing proteins (C1qDCs) with the galactose-binding lectin domain that is involved in P. marinus recognition, were only present and significantly upregulated in C. virginica. These results point to previously undescribed differences in host defense genes between the two oyster species that may account for the difference in susceptibility, providing an expanded portrait of the evolutionary dynamics of host-parasite interaction in lophotrochozoans that lack adaptive immunity. Our findings suggest that C. virginica and P. marinus have a history of coevolution and the recent outbreaks may be due to increased virulence of the parasite. Dataset Crassostrea gigas Pacific oyster Unknown Pacific |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation Jiulin Chan (3276780) Lu Wang (45927) Li Li (14993) Kang Mu (11787512) David Bushek (11787515) Yue Xu (246925) Ximing Guo (66272) Guofan Zhang (126339) Linlin Zhang (131810) Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation |
description |
Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is resistant to Dermo due to differences in the host-parasite interaction that is not well understood. We compared transcriptomic responses to P. marinus challenge in the two oysters at early and late infection stages. Dynamic and orchestrated regulation of large sets of innate immune response genes were observed in both species with remarkably similar patterns for most orthologs, although responses in C. virginica were stronger, suggesting strong or over-reacting immune response could be a cause of host mortality. Between the two species, several key immune response gene families differed in their expansion, sequence variation and/or transcriptional response to P. marinus, reflecting evolutionary divergence in host-parasite interaction. Of note, significant upregulation of inhibitors of apoptosis (IAPs) was observed in resistant C. gigas but not in susceptible C. virginica, suggesting upregulation of IAPs is an active defense mechanism, not a passive response orchestrated by P. marinus. Compared with C. gigas, C. virginica exhibited greater expansion of toll-like receptors (TLRs) and positive selection in P. marinus responsive TLRs. The C1q domain containing proteins (C1qDCs) with the galactose-binding lectin domain that is involved in P. marinus recognition, were only present and significantly upregulated in C. virginica. These results point to previously undescribed differences in host defense genes between the two oyster species that may account for the difference in susceptibility, providing an expanded portrait of the evolutionary dynamics of host-parasite interaction in lophotrochozoans that lack adaptive immunity. Our findings suggest that C. virginica and P. marinus have a history of coevolution and the recent outbreaks may be due to increased virulence of the parasite. |
format |
Dataset |
author |
Jiulin Chan (3276780) Lu Wang (45927) Li Li (14993) Kang Mu (11787512) David Bushek (11787515) Yue Xu (246925) Ximing Guo (66272) Guofan Zhang (126339) Linlin Zhang (131810) |
author_facet |
Jiulin Chan (3276780) Lu Wang (45927) Li Li (14993) Kang Mu (11787512) David Bushek (11787515) Yue Xu (246925) Ximing Guo (66272) Guofan Zhang (126339) Linlin Zhang (131810) |
author_sort |
Jiulin Chan (3276780) |
title |
Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
title_short |
Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
title_full |
Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
title_fullStr |
Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
title_full_unstemmed |
Table1_Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.DOCX |
title_sort |
table1_transcriptomic response to perkinsus marinus in two crassostrea oysters reveals evolutionary dynamics of host-parasite interactions.docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fgene.2021.795706.s004 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://figshare.com/articles/dataset/Table1_Transcriptomic_Response_to_Perkinsus_marinus_in_Two_Crassostrea_Oysters_Reveals_Evolutionary_Dynamics_of_Host-Parasite_Interactions_DOCX/17119886 doi:10.3389/fgene.2021.795706.s004 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fgene.2021.795706.s004 |
_version_ |
1766394916279156736 |