Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF
Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease. These strategies can be equally well applied to advance non-hu...
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Online Access: | https://doi.org/10.3389/fmolb.2021.778383.s011 |
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ftsmithonian:oai:figshare.com:article/16969483 2023-05-15T15:18:09+02:00 Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF Kazuhiro Aoki (56255) Tadahiro Kumagai (1910986) René Ranzinger (10052) Carl Bergmann (2096383) Alvin Camus (692679) Michael Tiemeyer (1348926) 2021-11-10T04:37:57Z https://doi.org/10.3389/fmolb.2021.778383.s011 unknown https://figshare.com/articles/figure/Image9_Serum_N-Glycome_Diversity_in_Teleost_and_Chondrostrean_Fishes_TIF/16969483 doi:10.3389/fmolb.2021.778383.s011 CC BY 4.0 CC-BY Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology glycomics mass spectrometry fish serum N-glycan Image Figure 2021 ftsmithonian https://doi.org/10.3389/fmolb.2021.778383.s011 2021-12-19T22:18:23Z Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease. These strategies can be equally well applied to advance non-human health care research. To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens. In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases. In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species. This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism. Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis. Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode. While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation. Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses. Still Image Arctic Atlantic salmon Human health Unknown Arctic |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology glycomics mass spectrometry fish serum N-glycan |
spellingShingle |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology glycomics mass spectrometry fish serum N-glycan Kazuhiro Aoki (56255) Tadahiro Kumagai (1910986) René Ranzinger (10052) Carl Bergmann (2096383) Alvin Camus (692679) Michael Tiemeyer (1348926) Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
topic_facet |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology glycomics mass spectrometry fish serum N-glycan |
description |
Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease. These strategies can be equally well applied to advance non-human health care research. To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens. In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases. In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species. This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism. Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis. Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode. While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation. Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses. |
format |
Still Image |
author |
Kazuhiro Aoki (56255) Tadahiro Kumagai (1910986) René Ranzinger (10052) Carl Bergmann (2096383) Alvin Camus (692679) Michael Tiemeyer (1348926) |
author_facet |
Kazuhiro Aoki (56255) Tadahiro Kumagai (1910986) René Ranzinger (10052) Carl Bergmann (2096383) Alvin Camus (692679) Michael Tiemeyer (1348926) |
author_sort |
Kazuhiro Aoki (56255) |
title |
Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
title_short |
Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
title_full |
Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
title_fullStr |
Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
title_full_unstemmed |
Image9_Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.TIF |
title_sort |
image9_serum n-glycome diversity in teleost and chondrostrean fishes.tif |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmolb.2021.778383.s011 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Atlantic salmon Human health |
genre_facet |
Arctic Atlantic salmon Human health |
op_relation |
https://figshare.com/articles/figure/Image9_Serum_N-Glycome_Diversity_in_Teleost_and_Chondrostrean_Fishes_TIF/16969483 doi:10.3389/fmolb.2021.778383.s011 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmolb.2021.778383.s011 |
_version_ |
1766348373594472448 |