Multidimensional Scaling (MDS) of genotyping programs.

• Main Authors: Isabel Salado (11604107), Alberto Fernández-Gil (578149), Carles Vilà (197062), Jennifer A. Leonard (9071318)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Genetics; Molecular Biology; Evolutionary Biology; Ecology; Biological Sciences not elsewhere classified; single multiplex reaction; reliable individual identification; relatively high number; new bioinformatic tools; low quality samples; iberian wolf (<; canis lupus </; software packages infer; recent software developments; four software packages; high quality genotype; ngs data may; generation sequencing data; div >< p; genotype microsatellite loci; 19 loci analyzed; automated microsatellite genotyping; microsatellite loci; generation sequencing; automated genotyping; genotyping process; time consuming; smaller amount; sequence reads; reasonable cost; provided genotypes; population characterization; pcr replicates; multiplexed reactions; laboratory work; kinship determination; increasing throughput; highly unoptimized; highly polymorphic; genotypes directly; generally short; first time; final analyses; fewer errors; fecal dna; endangered species; 10 replicates; Canis lupus
• Online Access: https://doi.org/10.1371/journal.pone.0258906.g001

Axes show distance values of distance matrix. Distance matrix was obtained from the comparison of consensus genotypes of each software and establishing minimum read depth of 16 (S2 Table in S1 File ). The closer two programs are, the more similar their results are. Model statistics: two-dimensions ratio MDS using majorization, stress-1 value (normalized): 0.061, number of iterations: 17. Reference indicates consensus genotypes used as reference for comparison.