Deactivation kinetics of melanopsin (Opn4) constructs.
A. In vitro Opn4 signaling kinetics based on normalized calcium imaging data of mouse Opn4 (blue trace), West Indian manatee (green trace), bowhead whale (purple trace), and mouse Opn4 lacking CTD phosphorylation sites (mouse phosphonull mutant—red trace). Negative control of untransfected HEK293 ce...
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ftsmithonian:oai:figshare.com:article/16820479 2023-05-15T15:45:58+02:00 Deactivation kinetics of melanopsin (Opn4) constructs. Jeffry I. Fasick (3624569) Haya Algrain (11570761) Courtland Samuels (11570764) Padmanabhan Mahadevan (475388) Lorian E. Schweikert (3624572) Zaid J. Naffaa (11570767) Phyllis R. Robinson (204369) 2021-10-15T17:31:17Z https://doi.org/10.1371/journal.pone.0257436.g008 unknown https://figshare.com/articles/figure/Deactivation_kinetics_of_melanopsin_Opn4_constructs_/16820479 doi:10.1371/journal.pone.0257436.g008 CC BY 4.0 CC-BY Biochemistry Cell Biology Molecular Biology Neuroscience Evolutionary Biology Ecology Virology Environmental Sciences not elsewhere classified water &# 8217 serve light detection retinal ganglion cells carboxyl terminal domain available light spectra marine mammal rhodopsins thus longer mydriasis extended pupillary constriction amino acid substitutions marine mammal species div >< p marine mammals may marine mammals pupil constriction mydriasis occurs identify substitutions given species values tuned values relative terrestrial counterparts terrestrial controls spectral tuning spectral sensitivity solar irradiance significant change rod retina rod monochromacy results suggest results show previous work prevent photobleaching orders sirenia likely involved infraorder cetacea highly photosensitive foraging depths deactivation kinetics condition known cdna sequences absorbance maxima Image Figure 2021 ftsmithonian https://doi.org/10.1371/journal.pone.0257436.g008 2021-12-20T00:06:07Z A. In vitro Opn4 signaling kinetics based on normalized calcium imaging data of mouse Opn4 (blue trace), West Indian manatee (green trace), bowhead whale (purple trace), and mouse Opn4 lacking CTD phosphorylation sites (mouse phosphonull mutant—red trace). Negative control of untransfected HEK293 cells (orange trace) is shown for comparison. B. Deactivation rates are significantly different between mouse Opn4 and the other three constructs. The rate of deactivation of West Indian manatee Opn4 is more similar to mouse Opn4, while the rate of deactivation of bowhead whale Opn4 is more similar to mouse phosphonull mutant. All rates were compared to the mouse Opn4 deactivation rate. *** and **** correspond to p-values of <0.0005 and <0.0001, respectively. Error bars denote standard error of the mean. Still Image bowhead whale Unknown Indian |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Biochemistry Cell Biology Molecular Biology Neuroscience Evolutionary Biology Ecology Virology Environmental Sciences not elsewhere classified water &# 8217 serve light detection retinal ganglion cells carboxyl terminal domain available light spectra marine mammal rhodopsins thus longer mydriasis extended pupillary constriction amino acid substitutions marine mammal species div >< p marine mammals may marine mammals pupil constriction mydriasis occurs identify substitutions given species values tuned values relative terrestrial counterparts terrestrial controls spectral tuning spectral sensitivity solar irradiance significant change rod retina rod monochromacy results suggest results show previous work prevent photobleaching orders sirenia likely involved infraorder cetacea highly photosensitive foraging depths deactivation kinetics condition known cdna sequences absorbance maxima |
spellingShingle |
Biochemistry Cell Biology Molecular Biology Neuroscience Evolutionary Biology Ecology Virology Environmental Sciences not elsewhere classified water &# 8217 serve light detection retinal ganglion cells carboxyl terminal domain available light spectra marine mammal rhodopsins thus longer mydriasis extended pupillary constriction amino acid substitutions marine mammal species div >< p marine mammals may marine mammals pupil constriction mydriasis occurs identify substitutions given species values tuned values relative terrestrial counterparts terrestrial controls spectral tuning spectral sensitivity solar irradiance significant change rod retina rod monochromacy results suggest results show previous work prevent photobleaching orders sirenia likely involved infraorder cetacea highly photosensitive foraging depths deactivation kinetics condition known cdna sequences absorbance maxima Jeffry I. Fasick (3624569) Haya Algrain (11570761) Courtland Samuels (11570764) Padmanabhan Mahadevan (475388) Lorian E. Schweikert (3624572) Zaid J. Naffaa (11570767) Phyllis R. Robinson (204369) Deactivation kinetics of melanopsin (Opn4) constructs. |
topic_facet |
Biochemistry Cell Biology Molecular Biology Neuroscience Evolutionary Biology Ecology Virology Environmental Sciences not elsewhere classified water &# 8217 serve light detection retinal ganglion cells carboxyl terminal domain available light spectra marine mammal rhodopsins thus longer mydriasis extended pupillary constriction amino acid substitutions marine mammal species div >< p marine mammals may marine mammals pupil constriction mydriasis occurs identify substitutions given species values tuned values relative terrestrial counterparts terrestrial controls spectral tuning spectral sensitivity solar irradiance significant change rod retina rod monochromacy results suggest results show previous work prevent photobleaching orders sirenia likely involved infraorder cetacea highly photosensitive foraging depths deactivation kinetics condition known cdna sequences absorbance maxima |
description |
A. In vitro Opn4 signaling kinetics based on normalized calcium imaging data of mouse Opn4 (blue trace), West Indian manatee (green trace), bowhead whale (purple trace), and mouse Opn4 lacking CTD phosphorylation sites (mouse phosphonull mutant—red trace). Negative control of untransfected HEK293 cells (orange trace) is shown for comparison. B. Deactivation rates are significantly different between mouse Opn4 and the other three constructs. The rate of deactivation of West Indian manatee Opn4 is more similar to mouse Opn4, while the rate of deactivation of bowhead whale Opn4 is more similar to mouse phosphonull mutant. All rates were compared to the mouse Opn4 deactivation rate. *** and **** correspond to p-values of <0.0005 and <0.0001, respectively. Error bars denote standard error of the mean. |
format |
Still Image |
author |
Jeffry I. Fasick (3624569) Haya Algrain (11570761) Courtland Samuels (11570764) Padmanabhan Mahadevan (475388) Lorian E. Schweikert (3624572) Zaid J. Naffaa (11570767) Phyllis R. Robinson (204369) |
author_facet |
Jeffry I. Fasick (3624569) Haya Algrain (11570761) Courtland Samuels (11570764) Padmanabhan Mahadevan (475388) Lorian E. Schweikert (3624572) Zaid J. Naffaa (11570767) Phyllis R. Robinson (204369) |
author_sort |
Jeffry I. Fasick (3624569) |
title |
Deactivation kinetics of melanopsin (Opn4) constructs. |
title_short |
Deactivation kinetics of melanopsin (Opn4) constructs. |
title_full |
Deactivation kinetics of melanopsin (Opn4) constructs. |
title_fullStr |
Deactivation kinetics of melanopsin (Opn4) constructs. |
title_full_unstemmed |
Deactivation kinetics of melanopsin (Opn4) constructs. |
title_sort |
deactivation kinetics of melanopsin (opn4) constructs. |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pone.0257436.g008 |
geographic |
Indian |
geographic_facet |
Indian |
genre |
bowhead whale |
genre_facet |
bowhead whale |
op_relation |
https://figshare.com/articles/figure/Deactivation_kinetics_of_melanopsin_Opn4_constructs_/16820479 doi:10.1371/journal.pone.0257436.g008 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0257436.g008 |
_version_ |
1766380636734488576 |