PCR cycling parameters.

• Main Authors: Jeffry I. Fasick (3624569), Haya Algrain (11570761), Courtland Samuels (11570764), Padmanabhan Mahadevan (475388), Lorian E. Schweikert (3624572), Zaid J. Naffaa (11570767), Phyllis R. Robinson (204369)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Biochemistry; Cell Biology; Molecular Biology; Neuroscience; Evolutionary Biology; Ecology; Virology; Environmental Sciences not elsewhere classified; water &# 8217; serve light detection; retinal ganglion cells; carboxyl terminal domain; available light spectra; marine mammal rhodopsins; thus longer mydriasis; extended pupillary constriction; amino acid substitutions; marine mammal species; div >< p; marine mammals may; marine mammals; pupil constriction; mydriasis occurs; identify substitutions; given species; values tuned; values relative; terrestrial counterparts; terrestrial controls; spectral tuning; spectral sensitivity; solar irradiance; significant change; rod retina; rod monochromacy; results suggest; results show; previous work; prevent photobleaching; orders sirenia; likely involved; infraorder cetacea; highly photosensitive; foraging depths; deactivation kinetics; condition known; cdna sequences; absorbance maxima; Indian; bowhead whale
• Online Access: https://doi.org/10.1371/journal.pone.0257436.s003

Temperatures for denaturation, annealing and extension for the amplification of West Indian manatee and bowhead whale opn4 coding sequences. (DOCX)