PCR cycling parameters.

• Main Authors: Jeffry I. Fasick (3624569), Haya Algrain (11570761), Courtland Samuels (11570764), Padmanabhan Mahadevan (475388), Lorian E. Schweikert (3624572), Zaid J. Naffaa (11570767), Phyllis R. Robinson (204369)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Biochemistry; Cell Biology; Molecular Biology; Neuroscience; Evolutionary Biology; Ecology; Virology; Environmental Sciences not elsewhere classified; water &# 8217; serve light detection; retinal ganglion cells; carboxyl terminal domain; available light spectra; marine mammal rhodopsins; thus longer mydriasis; extended pupillary constriction; amino acid substitutions; marine mammal species; div >< p; marine mammals may; marine mammals; pupil constriction; mydriasis occurs; identify substitutions; given species; values tuned; values relative; terrestrial counterparts; terrestrial controls; spectral tuning; spectral sensitivity; solar irradiance; significant change; rod retina; rod monochromacy; results suggest; results show; previous work; prevent photobleaching; orders sirenia; likely involved; infraorder cetacea; highly photosensitive; foraging depths; deactivation kinetics; condition known; cdna sequences; absorbance maxima; North Atlantic; North Atlantic right whale
• Online Access: https://doi.org/10.1371/journal.pone.0257436.s002

Temperatures for denaturation, annealing and extension for the amplification of Opn4 coding sequences from bottlenose dolphin, harbor porpoise and North Atlantic right whale retinal cDNA. Reactions contained forward and reverse primers (see above) and were carried out in a 25 μl mixture containing 1 μM of each primer, 2 μl of reverse transcribed single-stranded cDNA, and Amplitaq Gold master mix (Invitrogen, Carlsbad, CA). Annealing Tm were from NCBI Primer-BLAST for each primer pair. (DOCX)