PCR cycling parameters.
Temperatures for denaturation, annealing and extension for the amplification of Opn4 coding sequences from bottlenose dolphin, harbor porpoise and North Atlantic right whale retinal cDNA. Reactions contained forward and reverse primers (see above) and were carried out in a 25 μl mixture containing 1...
Main Authors: | , , , , , , |
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Format: | Other Non-Article Part of Journal/Newspaper |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0257436.s002 |
Summary: | Temperatures for denaturation, annealing and extension for the amplification of Opn4 coding sequences from bottlenose dolphin, harbor porpoise and North Atlantic right whale retinal cDNA. Reactions contained forward and reverse primers (see above) and were carried out in a 25 μl mixture containing 1 μM of each primer, 2 μl of reverse transcribed single-stranded cDNA, and Amplitaq Gold master mix (Invitrogen, Carlsbad, CA). Annealing Tm were from NCBI Primer-BLAST for each primer pair. (DOCX) |
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