Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been...
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ftsmithonian:oai:figshare.com:article/16782073 2023-05-15T13:43:46+02:00 Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX Timothy J. Williams (2321068) Michelle A. Allen (9224300) Jonathan F. Berengut (5334131) Ricardo Cavicchioli (431630) 2021-10-11T04:53:47Z https://doi.org/10.3389/fmicb.2021.741077.s005 unknown https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 doi:10.3389/fmicb.2021.741077.s005 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria Dataset 2021 ftsmithonian https://doi.org/10.3389/fmicb.2021.741077.s005 2021-12-20T00:32:27Z The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been identified in global anoxic environments, including (but not limited to) organic-carbon-rich lakes. Using 24 metagenome-assembled genomes (MAGs) obtained from an Antarctic lake (Ace Lake, Vestfold Hills), novel lineages and novel metabolic traits were identified for both phyla. The Cloacimonadota MAGs exhibited a capacity for carbon fixation using the reverse tricarboxylic acid cycle driven by oxidation of hydrogen and sulfur. Certain Cloacimonadota MAGs encoded proteins that possess dockerin and cohesin domains, which is consistent with the assembly of extracellular cellulosome-like structures that are used for degradation of polypeptides and polysaccharides. The Omnitrophota MAGs represented phylogenetically diverse taxa that were predicted to possess a strong biosynthetic capacity for amino acids, nucleosides, fatty acids, and essential cofactors. All of the Omnitrophota were inferred to be obligate fermentative heterotrophs that utilize a relatively narrow range of organic compounds, have an incomplete tricarboxylic acid cycle, and possess a single hydrogenase gene important for achieving redox balance in the cell. We reason that both Cloacimonadota and Omnitrophota form metabolic interactions with hydrogen-consuming partners (methanogens and Desulfobacterota, respectively) and, therefore, occupy specific niches in Ace Lake. Dataset Antarc* Antarctic Unknown Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Antarctic Vestfold Vestfold Hills |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria Timothy J. Williams (2321068) Michelle A. Allen (9224300) Jonathan F. Berengut (5334131) Ricardo Cavicchioli (431630) Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria |
description |
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been identified in global anoxic environments, including (but not limited to) organic-carbon-rich lakes. Using 24 metagenome-assembled genomes (MAGs) obtained from an Antarctic lake (Ace Lake, Vestfold Hills), novel lineages and novel metabolic traits were identified for both phyla. The Cloacimonadota MAGs exhibited a capacity for carbon fixation using the reverse tricarboxylic acid cycle driven by oxidation of hydrogen and sulfur. Certain Cloacimonadota MAGs encoded proteins that possess dockerin and cohesin domains, which is consistent with the assembly of extracellular cellulosome-like structures that are used for degradation of polypeptides and polysaccharides. The Omnitrophota MAGs represented phylogenetically diverse taxa that were predicted to possess a strong biosynthetic capacity for amino acids, nucleosides, fatty acids, and essential cofactors. All of the Omnitrophota were inferred to be obligate fermentative heterotrophs that utilize a relatively narrow range of organic compounds, have an incomplete tricarboxylic acid cycle, and possess a single hydrogenase gene important for achieving redox balance in the cell. We reason that both Cloacimonadota and Omnitrophota form metabolic interactions with hydrogen-consuming partners (methanogens and Desulfobacterota, respectively) and, therefore, occupy specific niches in Ace Lake. |
format |
Dataset |
author |
Timothy J. Williams (2321068) Michelle A. Allen (9224300) Jonathan F. Berengut (5334131) Ricardo Cavicchioli (431630) |
author_facet |
Timothy J. Williams (2321068) Michelle A. Allen (9224300) Jonathan F. Berengut (5334131) Ricardo Cavicchioli (431630) |
author_sort |
Timothy J. Williams (2321068) |
title |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_short |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_full |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_fullStr |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_full_unstemmed |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_sort |
table_5_shedding light on microbial “dark matter”: insights into novel cloacimonadota and omnitrophota from an antarctic lake.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.741077.s005 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake Antarctic Vestfold Vestfold Hills |
geographic_facet |
Ace Lake Antarctic Vestfold Vestfold Hills |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 doi:10.3389/fmicb.2021.741077.s005 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.741077.s005 |
_version_ |
1766192964936138752 |