Comparison of c-fos positive signal in the brain of fish exposed to different narrow bandwidth light.

• Main Authors: Mariann Eilertsen (11502317), Benjamin G. J. Clokie (11502320), Lars O. E. Ebbesson (2622343), Cristina Tanase (11502323), Herve Migaud (308426), Jon Vidar Helvik (8028887)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Cell Biology; Molecular Biology; Neuroscience; Physiology; Evolutionary Biology; Immunology; Developmental Biology; Science Policy; Mental Health; vertebrate ancient opsin; major receptive organs; emitting diodes technology; div >< p; putative opsin stimulation; potential direct activation; lateral tuberal nuclus; adapted salmon parr; stimulated neuronal activity; salmo salar </; photoreceptors expressing melanopsin; light sensory organs; melanopsin expressing cells; specific brain regions; similar brain regions; photosensitive brain regions; atlantic salmon (<; following light stimulation; deep brain photoreceptors; brain neural activity; activated following dark; neuronal modulation activity; overlapping expression patterns; neural activity; lateral cells; deep brain; atlantic salmon; light stimulation; neural activation; nonvisual photoreceptors; neuronal signals; melanopsin expression; teleost brain; specifically activated; salmon exposed; situ </; fos </; red light; visual world; various parts; suprachiasmatic nucleus; study aimed; strongest signal; pineal organ; pineal innervations; photoreceptive inputs; known presence; known marker; fish exposed; expression increased; either independent; different wavelengths; different spectra; apparent difference; 120 minutes; Atlantic salmon; Salmo salar
• Online Access: https://doi.org/10.1371/journal.pone.0258007.g004

Schematic drawings illustrate the plane of sections. A, E, I, M, Q, U: Control fish kept in darkness (Dark/Dark) showed no or little c-fos expression. B-D: A dorsal ring of c-fos positive cells was detected in the left habenula (hab) for all three spectra. F-H: In the ventral diencephalon, both the preoptic area (po) and superchiasmatic nucleus (scn) displayed c-fos expression. J-L: Expression of c-fos in the caudal habenula and dorsal thalamus (thd) for Blue (J) and Green (K), little expression in Red (L). N-P: Activated cells in caudal parts of the thalamus for all three spectra tested. R-T: In the hypothalamus, expression was detected in the anterior tuberal nucleus (nat) for the three spectra. In addition, a strong expression was detected in the lateral tuberal nucleus (nlt) for fish exposed to red light (T). Scale bars of 200 μm.