Activated brain regions after stimulation with white light for 120 minutes.

• Main Authors: Mariann Eilertsen (11502317), Benjamin G. J. Clokie (11502320), Lars O. E. Ebbesson (2622343), Cristina Tanase (11502323), Herve Migaud (308426), Jon Vidar Helvik (8028887)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Cell Biology; Molecular Biology; Neuroscience; Physiology; Evolutionary Biology; Immunology; Developmental Biology; Science Policy; Mental Health; vertebrate ancient opsin; major receptive organs; emitting diodes technology; div >< p; putative opsin stimulation; potential direct activation; lateral tuberal nuclus; adapted salmon parr; stimulated neuronal activity; salmo salar </; photoreceptors expressing melanopsin; light sensory organs; melanopsin expressing cells; specific brain regions; similar brain regions; photosensitive brain regions; atlantic salmon (<; following light stimulation; deep brain photoreceptors; brain neural activity; activated following dark; neuronal modulation activity; overlapping expression patterns; neural activity; lateral cells; deep brain; atlantic salmon; light stimulation; neural activation; nonvisual photoreceptors; neuronal signals; melanopsin expression; teleost brain; specifically activated; salmon exposed; situ </; fos </; red light; visual world; various parts; suprachiasmatic nucleus; study aimed; strongest signal; pineal organ; pineal innervations; photoreceptive inputs; known presence; known marker; fish exposed; expression increased; either independent; different wavelengths; different spectra; apparent difference; 120 minutes; Recess; Atlantic salmon; Salmo salar
• Online Access: https://doi.org/10.1371/journal.pone.0258007.g003

A1-E1: Nissl-stained transverse sections at the equivalent level of c-fos expressing cells illustrated by blue dots in salmon, parr. Schematic drawings illustrate the level of the section. A2-E2, A4-E4: The Nissl-stained cell populations of interest with a higher magnification. A3-E3, A5-E5 : c-fos expression at the same level and with the same high magnification. The overview illustrations (A1-E1) and the left sided tissues with c-fos expression (A3-E3, A5-E5) are presented by flipping the pictures, resembling a whole brain section. A3 : Expression in a dorsal ring in the left habenula (hab) and in ventral parts of the habenula. A5: c-fos expression in the suprachiasmatic nucleus (scn). B3: A cell group expressing c-fos just ventral to the caudal habenula. B5 : Expression in cells close to the third ventricle and in the magnocellular preoptic nucleus (pm). C3: Activated cells in the dorsal thalamus (thd) and ventral thalamus (thv) (posterior nucleus of thalamus) close to the third ventricle. C5 : Focus on the expression in dorsal thalamus (thd) where the expression was strongest. D3: Expression of c-fos in caudal parts of the thalamus. D5: In the hypothalamus, expression was seen in the anterior tuberal nucleus (nat) and lateral tuberal nucleus (nlt). E3: Cells expressing c-fos were also localized in the mesencephalic tectum (tect), longitudinal torus (tl) and semicircular torus (ts). E5: Expression in the nucleus of the lateral recess (nrl). Arrowheads indicate positive cells. Scale bars of 200 μm.