Activation of the immediate early gene c-fos after stimulation with white light was strongest after 120 minutes of light exposure.

• Main Authors: Mariann Eilertsen (11502317), Benjamin G. J. Clokie (11502320), Lars O. E. Ebbesson (2622343), Cristina Tanase (11502323), Herve Migaud (308426), Jon Vidar Helvik (8028887)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Cell Biology; Molecular Biology; Neuroscience; Physiology; Evolutionary Biology; Immunology; Developmental Biology; Science Policy; Mental Health; vertebrate ancient opsin; major receptive organs; emitting diodes technology; div >< p; putative opsin stimulation; potential direct activation; lateral tuberal nuclus; adapted salmon parr; stimulated neuronal activity; salmo salar </; photoreceptors expressing melanopsin; light sensory organs; melanopsin expressing cells; specific brain regions; similar brain regions; photosensitive brain regions; atlantic salmon (<; following light stimulation; deep brain photoreceptors; brain neural activity; activated following dark; neuronal modulation activity; overlapping expression patterns; neural activity; lateral cells; deep brain; atlantic salmon; light stimulation; neural activation; nonvisual photoreceptors; neuronal signals; melanopsin expression; teleost brain; specifically activated; salmon exposed; situ </; fos </; red light; visual world; various parts; suprachiasmatic nucleus; study aimed; strongest signal; pineal organ; pineal innervations; photoreceptive inputs; known presence; known marker; fish exposed; expression increased; either independent; different wavelengths; different spectra; apparent difference; 120 minutes; Atlantic salmon; Salmo salar
• Online Access: https://doi.org/10.1371/journal.pone.0258007.g002

In situ hybridization with c-fos is shown in the habenula (A, C, E, G, I) and thalamus (B, D, F, H, J) and schematic drawings indicate the plane of the cryo-section. A-B: Dark control, no c-fos expression detected. C-D: Sampling after 15 minutes exposure to white light, no c-fos expression detected. E-F: Sampling after 30 minutes exposure, weak c-fos expression detected. G-H: Sampling after 60 minutes exposure, strong c-fos expression detected. I-J: Sampling after 120 minutes exposure, the strongest c-fos expression detected. Scale bar of 200 μm.