Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx
Piscine orthoreovirus (PRV-1) infection causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus is also associated with focal melanized changes in white skeletal muscle where PRV-1 infection of macrophages appears to be important. In this study, we stu...
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ftsmithonian:oai:figshare.com:article/16625767 2023-05-15T15:31:27+02:00 Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx Muhammad Salman Malik (10718217) Ingvild Berg Nyman (10718223) Øystein Wessel (3772264) Maria K. Dahle (7352858) Espen Rimstad (248237) 2021-09-16T04:07:14Z https://doi.org/10.3389/fimmu.2021.729017.s012 unknown https://figshare.com/articles/dataset/Table_1_Dynamics_of_Polarized_Macrophages_and_Activated_CD8_Cells_in_Heart_Tissue_of_Atlantic_Salmon_Infected_With_Piscine_Orthoreovirus-1_xlsx/16625767 doi:10.3389/fimmu.2021.729017.s012 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon cell mediated immunity heart and skeletal muscle inflammation macrophage polarization Piscine orthoreovirus 1 Dataset 2021 ftsmithonian https://doi.org/10.3389/fimmu.2021.729017.s012 2021-12-20T02:09:28Z Piscine orthoreovirus (PRV-1) infection causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus is also associated with focal melanized changes in white skeletal muscle where PRV-1 infection of macrophages appears to be important. In this study, we studied the macrophage polarization into M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes during experimentally induced HSMI. The immune response in heart with HSMI lesions was characterized by CD8 + and MHC-I expressing cells and not by polarized macrophages. Fluorescent in situ hybridization (FISH) assays revealed localization of PRV-1 in a few M1 macrophages in both heart and skeletal muscle. M2 type macrophages were widely scattered in the heart and were more abundant in heart compared to the skeletal muscle. However, the M2 macrophages did not co-stain for PRV-1. There was a strong cellular immune response to the infection in the heart compared to that of the skeletal muscle, seen as increased MHC-I expression, partly in cells also containing PRV-1 RNA, and a high number of cytotoxic CD8 + granzyme producing cells that targeted PRV-1. In skeletal muscle, MHC-I expressing cells and CD8 + cells were dispersed between myocytes, but these cells did not stain for PRV-1. Gene expression analysis by RT-qPCR complied with the FISH results and confirmed a drop in level of PRV-1 following the cell mediated immune response. Overall, the results indicated that M1 macrophages do not contribute to the initial development of HSMI. However, large numbers of M2 macrophages reside in the heart and may contribute to the subsequent fast recovery following clearance of PRV-1 infection. Dataset Atlantic salmon Salmo salar Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon cell mediated immunity heart and skeletal muscle inflammation macrophage polarization Piscine orthoreovirus 1 |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon cell mediated immunity heart and skeletal muscle inflammation macrophage polarization Piscine orthoreovirus 1 Muhammad Salman Malik (10718217) Ingvild Berg Nyman (10718223) Øystein Wessel (3772264) Maria K. Dahle (7352858) Espen Rimstad (248237) Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon cell mediated immunity heart and skeletal muscle inflammation macrophage polarization Piscine orthoreovirus 1 |
description |
Piscine orthoreovirus (PRV-1) infection causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus is also associated with focal melanized changes in white skeletal muscle where PRV-1 infection of macrophages appears to be important. In this study, we studied the macrophage polarization into M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes during experimentally induced HSMI. The immune response in heart with HSMI lesions was characterized by CD8 + and MHC-I expressing cells and not by polarized macrophages. Fluorescent in situ hybridization (FISH) assays revealed localization of PRV-1 in a few M1 macrophages in both heart and skeletal muscle. M2 type macrophages were widely scattered in the heart and were more abundant in heart compared to the skeletal muscle. However, the M2 macrophages did not co-stain for PRV-1. There was a strong cellular immune response to the infection in the heart compared to that of the skeletal muscle, seen as increased MHC-I expression, partly in cells also containing PRV-1 RNA, and a high number of cytotoxic CD8 + granzyme producing cells that targeted PRV-1. In skeletal muscle, MHC-I expressing cells and CD8 + cells were dispersed between myocytes, but these cells did not stain for PRV-1. Gene expression analysis by RT-qPCR complied with the FISH results and confirmed a drop in level of PRV-1 following the cell mediated immune response. Overall, the results indicated that M1 macrophages do not contribute to the initial development of HSMI. However, large numbers of M2 macrophages reside in the heart and may contribute to the subsequent fast recovery following clearance of PRV-1 infection. |
format |
Dataset |
author |
Muhammad Salman Malik (10718217) Ingvild Berg Nyman (10718223) Øystein Wessel (3772264) Maria K. Dahle (7352858) Espen Rimstad (248237) |
author_facet |
Muhammad Salman Malik (10718217) Ingvild Berg Nyman (10718223) Øystein Wessel (3772264) Maria K. Dahle (7352858) Espen Rimstad (248237) |
author_sort |
Muhammad Salman Malik (10718217) |
title |
Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
title_short |
Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
title_full |
Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
title_fullStr |
Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
title_full_unstemmed |
Table_1_Dynamics of Polarized Macrophages and Activated CD8+ Cells in Heart Tissue of Atlantic Salmon Infected With Piscine Orthoreovirus-1.xlsx |
title_sort |
table_1_dynamics of polarized macrophages and activated cd8+ cells in heart tissue of atlantic salmon infected with piscine orthoreovirus-1.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.729017.s012 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://figshare.com/articles/dataset/Table_1_Dynamics_of_Polarized_Macrophages_and_Activated_CD8_Cells_in_Heart_Tissue_of_Atlantic_Salmon_Infected_With_Piscine_Orthoreovirus-1_xlsx/16625767 doi:10.3389/fimmu.2021.729017.s012 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.729017.s012 |
_version_ |
1766361959645577216 |