Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF
Neuropeptide Y (NPY) is known as a potent orexigenic signal in vertebrates, but its role in Atlantic salmon has not yet been fully established. In this study, we identified three npy paralogs, named npya1, npya2, and npyb, in the Atlantic salmon genome. In silico analysis revealed that these genes a...
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ftsmithonian:oai:figshare.com:article/16443558 2023-05-15T15:29:01+02:00 Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF Ingvill Tolås (11334690) Tharmini Kalananthan (7248713) Ana S. Gomes (7549852) Floriana Lai (4345858) Sissel Norland (11334693) Koji Murashita (7549856) Ivar Rønnestad (2622340) 2021-08-26T05:48:10Z https://doi.org/10.3389/fphys.2021.720639.s001 unknown https://figshare.com/articles/dataset/Data_Sheet_1_Regional_Expression_of_npy_mRNA_Paralogs_in_the_Brain_of_Atlantic_Salmon_Salmo_salar_L_and_Response_to_Fasting_PDF/16443558 doi:10.3389/fphys.2021.720639.s001 CC BY 4.0 CC-BY Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Atlantic salmon (Salmo salar L.) neuropeptide Y brain appetite control fullness fasting Dataset 2021 ftsmithonian https://doi.org/10.3389/fphys.2021.720639.s001 2021-12-20T03:33:38Z Neuropeptide Y (NPY) is known as a potent orexigenic signal in vertebrates, but its role in Atlantic salmon has not yet been fully established. In this study, we identified three npy paralogs, named npya1, npya2, and npyb, in the Atlantic salmon genome. In silico analysis revealed that these genes are well conserved across the vertebrate’s lineage and the mature peptide sequences shared at least 77% of identity with the human homolog. We analyzed mRNA expression of npy paralogs in eight brain regions of Atlantic salmon post-smolt, and the effect of 4 days of fasting on the npy expression level. Results show that npya1 was the most abundant paralog, and was predominantly expressed in the telencephalon, followed by the midbrain and olfactory bulb. npya2 mRNA was highly abundant in hypothalamus and midbrain, while npyb was found to be highest expressed in the telencephalon, with low mRNA expression levels detected in all the other brain regions. 4 days of fasting resulted in a significant (p < 0.05) decrease of npya1 mRNA expression in the olfactory bulb, increased npya2 mRNA expression in the midbrain and decreased npyb mRNA expression in the pituitary. In the hypothalamus, the vertebrate appetite center, expression of the npy paralogs was not significantly affected by feeding status. However, we observed a trend of increased npya2 mRNA expression (p = 0.099) following 4 days of fasting. Altogether, our findings provide a solid basis for further research on appetite and energy metabolism in Atlantic salmon. Dataset Atlantic salmon Salmo salar Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Atlantic salmon (Salmo salar L.) neuropeptide Y brain appetite control fullness fasting |
spellingShingle |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Atlantic salmon (Salmo salar L.) neuropeptide Y brain appetite control fullness fasting Ingvill Tolås (11334690) Tharmini Kalananthan (7248713) Ana S. Gomes (7549852) Floriana Lai (4345858) Sissel Norland (11334693) Koji Murashita (7549856) Ivar Rønnestad (2622340) Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
topic_facet |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Atlantic salmon (Salmo salar L.) neuropeptide Y brain appetite control fullness fasting |
description |
Neuropeptide Y (NPY) is known as a potent orexigenic signal in vertebrates, but its role in Atlantic salmon has not yet been fully established. In this study, we identified three npy paralogs, named npya1, npya2, and npyb, in the Atlantic salmon genome. In silico analysis revealed that these genes are well conserved across the vertebrate’s lineage and the mature peptide sequences shared at least 77% of identity with the human homolog. We analyzed mRNA expression of npy paralogs in eight brain regions of Atlantic salmon post-smolt, and the effect of 4 days of fasting on the npy expression level. Results show that npya1 was the most abundant paralog, and was predominantly expressed in the telencephalon, followed by the midbrain and olfactory bulb. npya2 mRNA was highly abundant in hypothalamus and midbrain, while npyb was found to be highest expressed in the telencephalon, with low mRNA expression levels detected in all the other brain regions. 4 days of fasting resulted in a significant (p < 0.05) decrease of npya1 mRNA expression in the olfactory bulb, increased npya2 mRNA expression in the midbrain and decreased npyb mRNA expression in the pituitary. In the hypothalamus, the vertebrate appetite center, expression of the npy paralogs was not significantly affected by feeding status. However, we observed a trend of increased npya2 mRNA expression (p = 0.099) following 4 days of fasting. Altogether, our findings provide a solid basis for further research on appetite and energy metabolism in Atlantic salmon. |
format |
Dataset |
author |
Ingvill Tolås (11334690) Tharmini Kalananthan (7248713) Ana S. Gomes (7549852) Floriana Lai (4345858) Sissel Norland (11334693) Koji Murashita (7549856) Ivar Rønnestad (2622340) |
author_facet |
Ingvill Tolås (11334690) Tharmini Kalananthan (7248713) Ana S. Gomes (7549852) Floriana Lai (4345858) Sissel Norland (11334693) Koji Murashita (7549856) Ivar Rønnestad (2622340) |
author_sort |
Ingvill Tolås (11334690) |
title |
Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
title_short |
Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
title_full |
Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
title_fullStr |
Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
title_full_unstemmed |
Data_Sheet_1_Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon (Salmo salar, L.) and Response to Fasting.PDF |
title_sort |
data_sheet_1_regional expression of npy mrna paralogs in the brain of atlantic salmon (salmo salar, l.) and response to fasting.pdf |
publishDate |
2021 |
url |
https://doi.org/10.3389/fphys.2021.720639.s001 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://figshare.com/articles/dataset/Data_Sheet_1_Regional_Expression_of_npy_mRNA_Paralogs_in_the_Brain_of_Atlantic_Salmon_Salmo_salar_L_and_Response_to_Fasting_PDF/16443558 doi:10.3389/fphys.2021.720639.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2021.720639.s001 |
_version_ |
1766359395173662720 |