Additional file 2 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing

• Main Authors: Henrik Christiansen (5717978), Franz M. Heindler (5717981), Bart Hellemans (3246426), Quentin Jossart (532157), Francesca Pasotti (5665906), Henri Robert (6970418), Marie Verheye (11309976), Bruno Danis (811532), Marc Kochzius (242381), Frederik Leliaert (186222), Camille Moreau (811531), Tasnim Patel (11309979), Anton P. Van de Putte (11309982), Ann Vanreusel (220781), Filip A. M. Volckaert (8140614), Isa Schön (153646)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Genetics; Biodiversity; Genome scan; Genotyping by sequencing; In silico digestion; RADseq; Southern Ocean
• Online Access: https://doi.org/10.6084/m9.figshare.16305498.v1

Additional file 2. In silico estimates of the number of fragments. Estimates were produced through in silico restriction enzyme digestions for reduced representation sequencing (RRS) optimized for approximately 30× coverage. The number of fragments depends on the restriction enzyme/combination, the size window, the assumed genome size, and the reference genome used for in silico digestion. Reference genomes of related species were used as well as simulated genomes; in this case the size and GC content used to simulate the genomes are listed. The number of fragments were extrapolated to the assumed genome size. Only two different enzyme and size selection setups per target species are listed here (for RRS setups optimized for HiSeq 2500 or HiSeq 4000 sequencing runs, respectively; the same as in Table 4, Table 5, Additional File 4); further estimates can be found in spreadsheets available at https://doi.org/10.5281/zenodo.5045574 .