Additional file 1 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing

• Main Authors: Henrik Christiansen (5717978), Franz M. Heindler (5717981), Bart Hellemans (3246426), Quentin Jossart (532157), Francesca Pasotti (5665906), Henri Robert (6970418), Marie Verheye (11309976), Bruno Danis (811532), Marc Kochzius (242381), Frederik Leliaert (186222), Camille Moreau (811531), Tasnim Patel (11309979), Anton P. Van de Putte (11309982), Ann Vanreusel (220781), Filip A. M. Volckaert (8140614), Isa Schön (153646)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Genetics; Biodiversity; Genome scan; Genotyping by sequencing; In silico digestion; RADseq; Southern Ocean
• Online Access: https://doi.org/10.6084/m9.figshare.16305495.v1

Additional file 1. Samples used for reduced representation sequencing (RRS) optimization. DNA from these samples was used for empirical restriction enzyme digestions with different enzymes (single digest EcoRI, PstI, MspI, or double digest EcoRI-MspI) and for RRS pilot libraries. Some samples were extracted twice as replicates (marked as _rep in sample ID). Three samples per species (family in the case of ostracods) were used for empirical digestions. The amphipod (C. obesa and E. pontomedon) samples and one T. loennbergii were used for empirical digestions, but not included in any RRS library.