Additional file 3 of Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

• Main Authors: Anne Hege Straume (8720607), Erik Kjærner-Semb (823939), Kai Ove Skaftnesmo (11174451), Hilal Güralp (11174454), Simon Lillico (6164513), Anna Wargelius (634280), Rolf Brudvik Edvardsen (9517013)
• Format: Conference Object
• Language: unknown
• Published: 2021
• Subjects: Genetics; HDR; ODN; Gene editing; Knock-in; Aquaculture; New breeding technologies; Straume; Atlantic salmon
• Online Access: https://doi.org/10.6084/m9.figshare.15041175.v1

Additional file 3: Figure S2. Comparison of symmetrical and asymmetrical ODNs for slc45a2 FLAG knock-in. All the ODNs compared here were designed for slc45a2 to KI a FLAG element. The ODN concentration was 1.5 μM. The symmetrical ODNs are a pool of S 24, AS 24, ds 24, S 48 and S 84 (described in Straume et.al 2020). The asymmetrical ODN design is illustrated in Suplemmentary Fig. 1 A. Green dots represent perfect HDR, black squares represent erroneous HDR. Mutant fish were analysed using Illumina MiSeq. Read counts for each sample are given in % of the total number of reads with at least 100 identical reads. The error bars indicate the SEM of the mean for each group. A Mann-Whitney test was used to compare the mean rank of symmetrical vs. asymmetrical ODNs, analyzing the groups perfect and erroneous HDR separately. Different lower-case letters indicate significant differences (P < 0.05).