DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx
Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interfer...
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Online Access: | https://doi.org/10.3389/fimmu.2021.696803.s001 |
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ftsmithonian:oai:figshare.com:article/14844489 2023-05-15T15:31:07+02:00 DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx Alvaro Santibañez (11025117) Diego Paine (11025120) Mick Parra (11025123) Carlos Muñoz (3147006) Natalia Valdes (3489596) Claudia Zapata (2421004) Rodrigo Vargas (731512) Alex Gonzalez (3489584) Mario Tello (657157) 2021-06-25T05:53:10Z https://doi.org/10.3389/fimmu.2021.696803.s001 unknown https://figshare.com/articles/dataset/DataSheet_1_Oral_Administration_of_Lactococcus_lactis_Producing_Interferon_Type_II_Enhances_the_Immune_Response_Against_Bacterial_Pathogens_in_Rainbow_Trout_docx/14844489 doi:10.3389/fimmu.2021.696803.s001 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Lactococcus lactis interferon type II Flavobacterium psychrophilum salmonids oral administration Dataset 2021 ftsmithonian https://doi.org/10.3389/fimmu.2021.696803.s001 2021-07-01T08:59:18Z Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interferon in Atlantic salmon activates the antiviral response, protecting against viral infection, but its role against bacterial infection has not been tested in vivo. In this work, through the design of a recombinant lactic acid bacterium capable of producing Interferon gamma from Atlantic salmon, we explore its role against bacterial infection and the ability to stimulate systemic immune response after oral administration of the recombinant probiotic. Recombinant interferon was active in vitro, mainly stimulating IL-6 expression in SHK-1 cells. In vivo, oral administration of the recombinant probiotic produced an increase in IL-6, IFNγ and IL-12 in the spleen and kidney, in addition to stimulating the activity of lysozyme in serum. The challenge trials indicated that the administration of the IFNγ-producing probiotic doubled the survival in fish infected with F. psychrophilum. In conclusion, our results showed that the oral administration of lactic acid bacteria producing IFNγ managed to stimulate the immune response at a systemic level, conferring protection against pathogens, showing a biotechnological potential for its application in aquaculture. Dataset Atlantic salmon Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Lactococcus lactis interferon type II Flavobacterium psychrophilum salmonids oral administration |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Lactococcus lactis interferon type II Flavobacterium psychrophilum salmonids oral administration Alvaro Santibañez (11025117) Diego Paine (11025120) Mick Parra (11025123) Carlos Muñoz (3147006) Natalia Valdes (3489596) Claudia Zapata (2421004) Rodrigo Vargas (731512) Alex Gonzalez (3489584) Mario Tello (657157) DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Lactococcus lactis interferon type II Flavobacterium psychrophilum salmonids oral administration |
description |
Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interferon in Atlantic salmon activates the antiviral response, protecting against viral infection, but its role against bacterial infection has not been tested in vivo. In this work, through the design of a recombinant lactic acid bacterium capable of producing Interferon gamma from Atlantic salmon, we explore its role against bacterial infection and the ability to stimulate systemic immune response after oral administration of the recombinant probiotic. Recombinant interferon was active in vitro, mainly stimulating IL-6 expression in SHK-1 cells. In vivo, oral administration of the recombinant probiotic produced an increase in IL-6, IFNγ and IL-12 in the spleen and kidney, in addition to stimulating the activity of lysozyme in serum. The challenge trials indicated that the administration of the IFNγ-producing probiotic doubled the survival in fish infected with F. psychrophilum. In conclusion, our results showed that the oral administration of lactic acid bacteria producing IFNγ managed to stimulate the immune response at a systemic level, conferring protection against pathogens, showing a biotechnological potential for its application in aquaculture. |
format |
Dataset |
author |
Alvaro Santibañez (11025117) Diego Paine (11025120) Mick Parra (11025123) Carlos Muñoz (3147006) Natalia Valdes (3489596) Claudia Zapata (2421004) Rodrigo Vargas (731512) Alex Gonzalez (3489584) Mario Tello (657157) |
author_facet |
Alvaro Santibañez (11025117) Diego Paine (11025120) Mick Parra (11025123) Carlos Muñoz (3147006) Natalia Valdes (3489596) Claudia Zapata (2421004) Rodrigo Vargas (731512) Alex Gonzalez (3489584) Mario Tello (657157) |
author_sort |
Alvaro Santibañez (11025117) |
title |
DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
title_short |
DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
title_full |
DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
title_fullStr |
DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
title_full_unstemmed |
DataSheet_1_Oral Administration of Lactococcus lactis Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.docx |
title_sort |
datasheet_1_oral administration of lactococcus lactis producing interferon type ii, enhances the immune response against bacterial pathogens in rainbow trout.docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.696803.s001 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://figshare.com/articles/dataset/DataSheet_1_Oral_Administration_of_Lactococcus_lactis_Producing_Interferon_Type_II_Enhances_the_Immune_Response_Against_Bacterial_Pathogens_in_Rainbow_Trout_docx/14844489 doi:10.3389/fimmu.2021.696803.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.696803.s001 |
_version_ |
1766361602510028800 |