Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx
Objectives Methylmercury metabolism was investigated in Pacific walruses (Odobenus rosmarus divergens) from St. Lawrence Island, Alaska, United States. Methods Total mercury and methylmercury concentrations were measured in fecal samples and paired colon samples (n = 16 walruses). Gut microbiota com...
Main Authors: | , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2021.648685.s001 |
id |
ftsmithonian:oai:figshare.com:article/14753103 |
---|---|
record_format |
openpolar |
spelling |
ftsmithonian:oai:figshare.com:article/14753103 2023-05-15T15:12:37+02:00 Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx Sarah E. Rothenberg (2427184) Danielle N. Sweitzer (10944987) Bryna R. Rackerby (10944990) Claire E. Couch (4002761) Lesley A. Cohen (10944993) Heather M. Broughton (10944996) Sheanna M. Steingass (10944999) Brianna R. Beechler (4002764) 2021-06-09T05:19:22Z https://doi.org/10.3389/fmicb.2021.648685.s001 unknown https://figshare.com/articles/dataset/Data_Sheet_1_Fecal_Methylmercury_Correlates_With_Gut_Microbiota_Taxa_in_Pacific_Walruses_Odobenus_rosmarus_divergens_docx/14753103 doi:10.3389/fmicb.2021.648685.s001 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology marine mammal metabolism Arctic pinniped microbiome colon alpha diversity stable isotopes of C and N Dataset 2021 ftsmithonian https://doi.org/10.3389/fmicb.2021.648685.s001 2021-06-13T14:50:30Z Objectives Methylmercury metabolism was investigated in Pacific walruses (Odobenus rosmarus divergens) from St. Lawrence Island, Alaska, United States. Methods Total mercury and methylmercury concentrations were measured in fecal samples and paired colon samples (n = 16 walruses). Gut microbiota composition and diversity were determined using 16S rRNA gene sequencing. Associations between fecal and colon mercury and the 24 most prevalent gut microbiota taxa were investigated using linear models. Results In fecal samples, the median values for total mercury, methylmercury, and %methylmercury (of total mercury) were 200 ng/g, 4.7 ng/g, and 2.5%, respectively, while in colon samples, the median values for the same parameters were 28 ng/g, 7.8 ng/g, and 26%, respectively. In fecal samples, methylmercury was negatively correlated with one Bacteroides genus, while members of the Oscillospirales order were positively correlated with both methylmercury and %methylmercury (of total mercury). In colon samples, %methylmercury (of total mercury) was negatively correlated with members of two genera, Romboutsia and Paeniclostridium. Conclusions Median %methylmercury (of total mercury) was 10 times higher in the colon compared to the fecal samples, suggesting that methylmercury was able to pass through the colon into systemic circulation. Fecal total mercury and/or methylmercury concentrations in walruses were comparable to some human studies despite differences in seafood consumption rates, suggesting that walruses excreted less mercury. There are no members (at this time) of the Oscillospirales order which are known to contain the genes to methylate mercury, suggesting the source of methylmercury in the gut was from diet and not in vivo methylation. Dataset Arctic Odobenus rosmarus St Lawrence Island Alaska walrus* Unknown Arctic Pacific Lawrence Island ENVELOPE(-103.718,-103.718,56.967,56.967) |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology marine mammal metabolism Arctic pinniped microbiome colon alpha diversity stable isotopes of C and N |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology marine mammal metabolism Arctic pinniped microbiome colon alpha diversity stable isotopes of C and N Sarah E. Rothenberg (2427184) Danielle N. Sweitzer (10944987) Bryna R. Rackerby (10944990) Claire E. Couch (4002761) Lesley A. Cohen (10944993) Heather M. Broughton (10944996) Sheanna M. Steingass (10944999) Brianna R. Beechler (4002764) Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology marine mammal metabolism Arctic pinniped microbiome colon alpha diversity stable isotopes of C and N |
description |
Objectives Methylmercury metabolism was investigated in Pacific walruses (Odobenus rosmarus divergens) from St. Lawrence Island, Alaska, United States. Methods Total mercury and methylmercury concentrations were measured in fecal samples and paired colon samples (n = 16 walruses). Gut microbiota composition and diversity were determined using 16S rRNA gene sequencing. Associations between fecal and colon mercury and the 24 most prevalent gut microbiota taxa were investigated using linear models. Results In fecal samples, the median values for total mercury, methylmercury, and %methylmercury (of total mercury) were 200 ng/g, 4.7 ng/g, and 2.5%, respectively, while in colon samples, the median values for the same parameters were 28 ng/g, 7.8 ng/g, and 26%, respectively. In fecal samples, methylmercury was negatively correlated with one Bacteroides genus, while members of the Oscillospirales order were positively correlated with both methylmercury and %methylmercury (of total mercury). In colon samples, %methylmercury (of total mercury) was negatively correlated with members of two genera, Romboutsia and Paeniclostridium. Conclusions Median %methylmercury (of total mercury) was 10 times higher in the colon compared to the fecal samples, suggesting that methylmercury was able to pass through the colon into systemic circulation. Fecal total mercury and/or methylmercury concentrations in walruses were comparable to some human studies despite differences in seafood consumption rates, suggesting that walruses excreted less mercury. There are no members (at this time) of the Oscillospirales order which are known to contain the genes to methylate mercury, suggesting the source of methylmercury in the gut was from diet and not in vivo methylation. |
format |
Dataset |
author |
Sarah E. Rothenberg (2427184) Danielle N. Sweitzer (10944987) Bryna R. Rackerby (10944990) Claire E. Couch (4002761) Lesley A. Cohen (10944993) Heather M. Broughton (10944996) Sheanna M. Steingass (10944999) Brianna R. Beechler (4002764) |
author_facet |
Sarah E. Rothenberg (2427184) Danielle N. Sweitzer (10944987) Bryna R. Rackerby (10944990) Claire E. Couch (4002761) Lesley A. Cohen (10944993) Heather M. Broughton (10944996) Sheanna M. Steingass (10944999) Brianna R. Beechler (4002764) |
author_sort |
Sarah E. Rothenberg (2427184) |
title |
Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
title_short |
Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
title_full |
Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
title_fullStr |
Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
title_full_unstemmed |
Data_Sheet_1_Fecal Methylmercury Correlates With Gut Microbiota Taxa in Pacific Walruses (Odobenus rosmarus divergens).docx |
title_sort |
data_sheet_1_fecal methylmercury correlates with gut microbiota taxa in pacific walruses (odobenus rosmarus divergens).docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.648685.s001 |
long_lat |
ENVELOPE(-103.718,-103.718,56.967,56.967) |
geographic |
Arctic Pacific Lawrence Island |
geographic_facet |
Arctic Pacific Lawrence Island |
genre |
Arctic Odobenus rosmarus St Lawrence Island Alaska walrus* |
genre_facet |
Arctic Odobenus rosmarus St Lawrence Island Alaska walrus* |
op_relation |
https://figshare.com/articles/dataset/Data_Sheet_1_Fecal_Methylmercury_Correlates_With_Gut_Microbiota_Taxa_in_Pacific_Walruses_Odobenus_rosmarus_divergens_docx/14753103 doi:10.3389/fmicb.2021.648685.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.648685.s001 |
_version_ |
1766343277881065472 |