SDS-PAGE analysis of the purified PaE.

MW, molecular weight marker (Precision Plus Protein Unstained Standards, Bio-Rad Laboratories Inc.); 1, culture filtrate of Pseudozyma antarctica X-14 strain; 2, purified PaE that was eluted from a Mono S column (GE Healthcare Life Sciences) using a linear gradient of 0–0.2 M NaCl and concentrated w...

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Bibliographic Details
Main Authors: Takumi Tanaka (1977604), Ken Suzuki (75835), Hirokazu Ueda (2478403), Yuka Sameshima-Yamashita (6292238), Hiroko Kitamoto (6076307)
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2021
Subjects:
Biochemistry
Microbiology
Biotechnology
Ecology
Science Policy
Environmental Sciences not elsewhere classified
Astronomical and Space Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
basidiomycete yeast Pseudozyma anta.
ethanol precipitation steps
concentration
decreases ethanol use
PaE
ethanol treatment
Pseudozyma antarctica culture
sequential ammonium sulfate precipi.
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0252811.s003
Description
Summary:MW, molecular weight marker (Precision Plus Protein Unstained Standards, Bio-Rad Laboratories Inc.); 1, culture filtrate of Pseudozyma antarctica X-14 strain; 2, purified PaE that was eluted from a Mono S column (GE Healthcare Life Sciences) using a linear gradient of 0–0.2 M NaCl and concentrated with an Amicon Ultra Centrifugal Filter (pore size: 3 kDa; Merck KGaA). (DOCX)

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