Understanding Design Rules for Optimizing the Interface between Immobilized Enzymes and Random Copolymer Brushes

• Main Authors: Héctor Sánchez-Morán (7817531), James S. Weltz (8837648), Daniel K. Schwartz (1303779), Joel L. Kaar (124911)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Biochemistry; Genetics; Biotechnology; Inorganic Chemistry; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; Information Systems not elsewhere classified; PEGMA; aliphatic residues; material; Candida antarctica lipase B; Design Rules; enzyme surface area; Bacillus subtilis lipase; Immobilized Enzymes; brush composition; Candida rugosa lipase; SBMA; unsupervised cluster analysis; ruggedize enzymes; Random Copolymer Brushes; sulfobetaine methacrylate; brush layer; copolymer brush surfaces; protein surface hydrophobicity; CALB; Rugosa; Antarc*; Antarctica
• Online Access: https://doi.org/10.1021/acsami.1c02443.s001

A long-standing goal in the field of biotechnology is to develop and understand design rules for the stabilization of enzymes upon immobilization to materials. While immobilization has sometimes been successful as a strategy to stabilize enzymes, the design of synthetic materials that stabilize enzy...