Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif
Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response...
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Online Access: | https://doi.org/10.3389/fimmu.2021.666356.s001 |
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ftsmithonian:oai:figshare.com:article/14584998 2023-05-15T15:32:04+02:00 Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif Byron Morales-Lange (5337284) Felipe Ramírez-Cepeda (10792866) Paulina Schmitt (463501) Fanny Guzmán (3180201) Leidy Lagos (6305471) Margareth Øverland (502788) Valentina Wong-Benito (10792869) Mónica Imarai (763203) Derie Fuentes (10792872) Sebastián Boltaña (5660179) Javier Alcaíno (10792875) Carlos Soto (3270231) Luis Mercado (685871) 2021-05-13T05:36:22Z https://doi.org/10.3389/fimmu.2021.666356.s001 unknown https://figshare.com/articles/figure/Image_1_Interferon_Gamma_Induces_the_Increase_of_Cell-Surface_Markers_CD80_86_CD83_and_MHC-II_in_Splenocytes_From_Atlantic_Salmon_tif/14584998 doi:10.3389/fimmu.2021.666356.s001 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmo salar fish interferon-gamma fish dendritic cells fish splenocytes fish phenotypic markers fish zbtb46 Image Figure 2021 ftsmithonian https://doi.org/10.3389/fimmu.2021.666356.s001 2021-05-21T14:59:53Z Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response and enhances the production of nitrogen and oxygen reactive species in phagocytic cells. This work studied the effect of IFNγ on the expression of cell-surface markers on splenocytes of Atlantic salmon (Salmo salar). In vitro results showed that subpopulations of mononuclear splenocytes cultured for 15 days were capable of increasing gene expression and protein availability of cell-surface markers such as CD80/86, CD83 and MHC II, after being stimulated with recombinant IFNγ. These results were observed for subpopulations with characteristics associated with monocytes (51%), and features that could be related to lymphocytes (46.3%). In addition, a decrease in the expression of zbtb46 was detected in IFNγ-stimulated splenocytes. Finally, the expression of IFNγ and cell-surface markers was assessed in Atlantic salmon under field conditions. In vivo results showed that the expression of ifnγ increased simultaneously with the up-regulation of cd80/86, cd83 and mhcii during a natural outbreak of Piscirickettsia salmonis. Overall, the results obtained in this study allow us to propose IFNγ as a candidate molecule to stimulate the phenotypic progression of a small population of immune cells, which will increase antigen presenting cells markers. Thereby, modulatory strategies using IFNγ may generate a robust and coordinated immune response in fish against pathogens that affect aquaculture. Still Image Atlantic salmon Salmo salar Unknown |
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ftsmithonian |
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topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmo salar fish interferon-gamma fish dendritic cells fish splenocytes fish phenotypic markers fish zbtb46 |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmo salar fish interferon-gamma fish dendritic cells fish splenocytes fish phenotypic markers fish zbtb46 Byron Morales-Lange (5337284) Felipe Ramírez-Cepeda (10792866) Paulina Schmitt (463501) Fanny Guzmán (3180201) Leidy Lagos (6305471) Margareth Øverland (502788) Valentina Wong-Benito (10792869) Mónica Imarai (763203) Derie Fuentes (10792872) Sebastián Boltaña (5660179) Javier Alcaíno (10792875) Carlos Soto (3270231) Luis Mercado (685871) Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmo salar fish interferon-gamma fish dendritic cells fish splenocytes fish phenotypic markers fish zbtb46 |
description |
Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response and enhances the production of nitrogen and oxygen reactive species in phagocytic cells. This work studied the effect of IFNγ on the expression of cell-surface markers on splenocytes of Atlantic salmon (Salmo salar). In vitro results showed that subpopulations of mononuclear splenocytes cultured for 15 days were capable of increasing gene expression and protein availability of cell-surface markers such as CD80/86, CD83 and MHC II, after being stimulated with recombinant IFNγ. These results were observed for subpopulations with characteristics associated with monocytes (51%), and features that could be related to lymphocytes (46.3%). In addition, a decrease in the expression of zbtb46 was detected in IFNγ-stimulated splenocytes. Finally, the expression of IFNγ and cell-surface markers was assessed in Atlantic salmon under field conditions. In vivo results showed that the expression of ifnγ increased simultaneously with the up-regulation of cd80/86, cd83 and mhcii during a natural outbreak of Piscirickettsia salmonis. Overall, the results obtained in this study allow us to propose IFNγ as a candidate molecule to stimulate the phenotypic progression of a small population of immune cells, which will increase antigen presenting cells markers. Thereby, modulatory strategies using IFNγ may generate a robust and coordinated immune response in fish against pathogens that affect aquaculture. |
format |
Still Image |
author |
Byron Morales-Lange (5337284) Felipe Ramírez-Cepeda (10792866) Paulina Schmitt (463501) Fanny Guzmán (3180201) Leidy Lagos (6305471) Margareth Øverland (502788) Valentina Wong-Benito (10792869) Mónica Imarai (763203) Derie Fuentes (10792872) Sebastián Boltaña (5660179) Javier Alcaíno (10792875) Carlos Soto (3270231) Luis Mercado (685871) |
author_facet |
Byron Morales-Lange (5337284) Felipe Ramírez-Cepeda (10792866) Paulina Schmitt (463501) Fanny Guzmán (3180201) Leidy Lagos (6305471) Margareth Øverland (502788) Valentina Wong-Benito (10792869) Mónica Imarai (763203) Derie Fuentes (10792872) Sebastián Boltaña (5660179) Javier Alcaíno (10792875) Carlos Soto (3270231) Luis Mercado (685871) |
author_sort |
Byron Morales-Lange (5337284) |
title |
Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
title_short |
Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
title_full |
Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
title_fullStr |
Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
title_full_unstemmed |
Image_1_Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon.tif |
title_sort |
image_1_interferon gamma induces the increase of cell-surface markers (cd80/86, cd83 and mhc-ii) in splenocytes from atlantic salmon.tif |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.666356.s001 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://figshare.com/articles/figure/Image_1_Interferon_Gamma_Induces_the_Increase_of_Cell-Surface_Markers_CD80_86_CD83_and_MHC-II_in_Splenocytes_From_Atlantic_Salmon_tif/14584998 doi:10.3389/fimmu.2021.666356.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.666356.s001 |
_version_ |
1766362565073436672 |