DNA methylation dynamics in B . hygrometrica plants during acclimation and responding to rapid dehydration stress.

• Main Authors: Run-Ze Sun (6080933), Jie Liu (15128), Yuan-Yuan Wang (349223), Xin Deng (186800)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Microbiology; Genetics; Molecular Biology; Ecology; Plant Biology; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; CHG; CHH; dehydration stress-responsive hypom.; gene body methylation levels; drought acclimation; DNA methylation-mediated modulation; context; Global transcriptome analysis; whole-genome DNA methylation; resurrection plant Boea hygrometric.; gene expression levels; drought acclimation-induced RDT; dehydration tolerance; CG; resurrection plant Boea hygrometrica; methylation pathway genes; pre-mRNA-splicing factor 38; Mild drought acclimation; dehydration stress-induced upregulation; dehydration stress response; promoter methylation variations; acid transporter 1-; post-translational protein modification; desiccation tolerance acquisition; dehydration stress memory; DML
• Online Access: https://doi.org/10.1371/journal.pgen.1009549.g003

(A) Expression profile of genes encoding DNA methylation and demethylation-related enzymes during drought acclimation, growth, and response to dehydration stress. MET1/CMT3, DNA methyltransferase 1/chromomethylase 3; HDAC, histone deacetylase; DNMT, DNA methyltransferase; DRM2, domains rearranged methyltransferase 2; DDM1, decrease in DNA methylation 1; RDM4, RNA-directed DNA methylation 4; FDM1, factor of DNA methylation 1; DML/ROS1, DEMETER-like DNA demethylase/repressor of silencing 1. Scale bar represents the normalized expression of each gene between samples. Genes with significant expression changes (fold change ≥ 2 and adjusted p ≤ 0.05) in drought-acclimated plants compared with the non-acclimated control groups (A/F, A4/F4, and A18/F18) and in plants subjected to dehydration stress (SD/F, FD/F, A/F, F4D/F4, A4D/A4, F18D/F18, and A18D/A18) are indicated by asterisks (*) in the corresponding squares. (B) Global changes in the average proportion of methylated C, CG, CHH, and CHG. (C) Correlation analysis between the level of DNA methylation and the expression of methylation pathway genes. (D) Number statistic of DMRs between samples. Hypo- and hyper-DMRs are shown in the left and right columns, respectively.