PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.

Wild-type and ΔPa PRO1 and ΔPa PRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fra...

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Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0247462.g002
id ftsmithonian:oai:figshare.com:article/14231005
record_format openpolar
spelling ftsmithonian:oai:figshare.com:article/14231005 2023-05-15T13:43:17+02:00 PaE production in ΔPa PRO1 and ΔPa PRO2 mutants. Natsuki Omae (10318504) Yuka Sameshima-Yamashita (6292238) Kazunori Ushimaru (3689119) Hideaki Koike (410323) Hiroko Kitamoto (6076307) Tomotake Morita (526538) 2021-03-17T17:38:25Z https://doi.org/10.1371/journal.pone.0247462.g002 unknown https://figshare.com/articles/figure/PaE_production_in_Pa_i_PRO1_i_and_Pa_i_PRO2_i_mutants_/14231005 doi:10.1371/journal.pone.0247462.g002 CC BY 4.0 CC-BY Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuolar protease function yeast Pseudozyma antarctica GB plastic-degrading enzyme 48 h incubation orthologous genes encoding proteases 3- L jar fermenter XG 8 XG 8 Δ Pa strain XG 8 control B orthologous genes PaE degradation fragments Δ Pa Image Figure 2021 ftsmithonian https://doi.org/10.1371/journal.pone.0247462.g002 2021-03-23T16:29:03Z Wild-type and ΔPa PRO1 and ΔPa PRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fragments of PaE in the strain GB-4(0). Still Image Antarc* Antarctica Unknown
institution Open Polar
collection Unknown
op_collection_id ftsmithonian
language unknown
topic Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
spellingShingle Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
topic_facet Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
description Wild-type and ΔPa PRO1 and ΔPa PRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fragments of PaE in the strain GB-4(0).
format Still Image
author Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_facet Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_sort Natsuki Omae (10318504)
title PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
title_short PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
title_full PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
title_fullStr PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
title_full_unstemmed PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.
title_sort pae production in δpa pro1 and δpa pro2 mutants.
publishDate 2021
url https://doi.org/10.1371/journal.pone.0247462.g002
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation https://figshare.com/articles/figure/PaE_production_in_Pa_i_PRO1_i_and_Pa_i_PRO2_i_mutants_/14231005
doi:10.1371/journal.pone.0247462.g002
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.1371/journal.pone.0247462.g002
_version_ 1766186969692372992

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