PaE production in ΔPa PRO1 and ΔPa PRO2 mutants.

Wild-type and ΔPa PRO1 and ΔPa PRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fra...

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Bibliographic Details
Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0247462.g002
Description
Summary:Wild-type and ΔPa PRO1 and ΔPa PRO2 gene-deletion mutants of strain GB-4(0) were cultured for 5 and 13 days. PaE and its degraded fragments in the supernatant were detected with anti-PaE antibody. PC, purified PaE as the positive control; black triangle, full-length PaE; gray triangles, degraded fragments of PaE in the strain GB-4(0).

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