Intracellular protease activity of ΔPa PRO1 , ΔPa PRO2 , and wild-type.

• Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Biochemistry; Microbiology; Cell Biology; Genetics; Molecular Biology; Developmental Biology; Science Policy; Infectious Diseases; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; vacuolar protease function; yeast Pseudozyma antarctica; GB; plastic-degrading enzyme; 48 h incubation; orthologous genes encoding proteases; 3- L jar fermenter; XG 8; XG 8 Δ Pa; strain; XG 8 control; B orthologous genes; PaE; degradation fragments; Δ Pa; St. Louis; Aldrich; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0247462.s013

The activity of extra- (A) and intracellular (B) acid proteases was determined in the same manner as published studies [ 14 , 36 ] with minor modifications. All strains were cultivated at 30°C for 5 days as with the flask cultivation in the Materials and methods section. The 1 mL of cultures after t...