Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip
In contrast to temperate systems, Arctic lagoons that span the Alaska Beaufort Sea coast face extreme seasonality. Nine months of ice cover up to ∼1.7 m thick is followed by a spring thaw that introduces an enormous pulse of freshwater, nutrients, and organic matter into these lagoons over a relativ...
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ftsmithonian:oai:figshare.com:article/13939019 2023-05-15T14:53:03+02:00 Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip Kristina D. Baker (4020629) Colleen T. E. Kellogg (8060999) James W. McClelland (2909585) Kenneth H. Dunton (8061002) Byron C. Crump (8061005) 2021-02-12T04:11:22Z https://doi.org/10.3389/fmicb.2021.601901.s001 unknown https://figshare.com/articles/dataset/Data_Sheet_1_The_Genomic_Capabilities_of_Microbial_Communities_Track_Seasonal_Variation_in_Environmental_Conditions_of_Arctic_Lagoons_zip/13939019 doi:10.3389/fmicb.2021.601901.s001 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology estuary archaea bacteria omics arctic national wildlife refuge Dataset 2021 ftsmithonian https://doi.org/10.3389/fmicb.2021.601901.s001 2021-02-26T11:35:54Z In contrast to temperate systems, Arctic lagoons that span the Alaska Beaufort Sea coast face extreme seasonality. Nine months of ice cover up to ∼1.7 m thick is followed by a spring thaw that introduces an enormous pulse of freshwater, nutrients, and organic matter into these lagoons over a relatively brief 2–3 week period. Prokaryotic communities link these subsidies to lagoon food webs through nutrient uptake, heterotrophic production, and other biogeochemical processes, but little is known about how the genomic capabilities of these communities respond to seasonal variability. Replicate water samples from two lagoons and one coastal site near Kaktovik, AK were collected in April (full ice cover), June (ice break up), and August (open water) to represent winter, spring, and summer, respectively. Samples were size fractionated to distinguish free-living and particle-attached microbial communities. Multivariate analysis of metagenomes indicated that seasonal variability in gene abundances was greater than variability between size fractions and sites, and that June differed significantly from the other months. Spring (June) gene abundances reflected the high input of watershed-sourced nutrients and organic matter via spring thaw, featuring indicator genes for denitrification possibly linked to greater organic carbon availability, and genes for processing phytoplankton-derived organic matter associated with spring blooms. Summer featured fewer indicator genes, but had increased abundances of anoxygenic photosynthesis genes, possibly associated with elevated light availability. Winter (April) gene abundances suggested low energy inputs and autotrophic bacterial metabolism, featuring indicator genes for chemoautotrophic carbon fixation, methane metabolism, and nitrification. Winter indicator genes for nitrification belonged to Thaumarchaeota and Nitrosomonadales, suggesting these organisms play an important role in oxidizing ammonium during the under-ice period. This study shows that high latitude estuarine microbial assemblages shift metabolic capabilities as they change phylogenetic composition between these extreme seasons, providing evidence that these communities may be resilient to large hydrological events in a rapidly changing Arctic. Dataset Arctic Beaufort Sea Phytoplankton Alaska Unknown Arctic |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology estuary archaea bacteria omics arctic national wildlife refuge |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology estuary archaea bacteria omics arctic national wildlife refuge Kristina D. Baker (4020629) Colleen T. E. Kellogg (8060999) James W. McClelland (2909585) Kenneth H. Dunton (8061002) Byron C. Crump (8061005) Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology estuary archaea bacteria omics arctic national wildlife refuge |
description |
In contrast to temperate systems, Arctic lagoons that span the Alaska Beaufort Sea coast face extreme seasonality. Nine months of ice cover up to ∼1.7 m thick is followed by a spring thaw that introduces an enormous pulse of freshwater, nutrients, and organic matter into these lagoons over a relatively brief 2–3 week period. Prokaryotic communities link these subsidies to lagoon food webs through nutrient uptake, heterotrophic production, and other biogeochemical processes, but little is known about how the genomic capabilities of these communities respond to seasonal variability. Replicate water samples from two lagoons and one coastal site near Kaktovik, AK were collected in April (full ice cover), June (ice break up), and August (open water) to represent winter, spring, and summer, respectively. Samples were size fractionated to distinguish free-living and particle-attached microbial communities. Multivariate analysis of metagenomes indicated that seasonal variability in gene abundances was greater than variability between size fractions and sites, and that June differed significantly from the other months. Spring (June) gene abundances reflected the high input of watershed-sourced nutrients and organic matter via spring thaw, featuring indicator genes for denitrification possibly linked to greater organic carbon availability, and genes for processing phytoplankton-derived organic matter associated with spring blooms. Summer featured fewer indicator genes, but had increased abundances of anoxygenic photosynthesis genes, possibly associated with elevated light availability. Winter (April) gene abundances suggested low energy inputs and autotrophic bacterial metabolism, featuring indicator genes for chemoautotrophic carbon fixation, methane metabolism, and nitrification. Winter indicator genes for nitrification belonged to Thaumarchaeota and Nitrosomonadales, suggesting these organisms play an important role in oxidizing ammonium during the under-ice period. This study shows that high latitude estuarine microbial assemblages shift metabolic capabilities as they change phylogenetic composition between these extreme seasons, providing evidence that these communities may be resilient to large hydrological events in a rapidly changing Arctic. |
format |
Dataset |
author |
Kristina D. Baker (4020629) Colleen T. E. Kellogg (8060999) James W. McClelland (2909585) Kenneth H. Dunton (8061002) Byron C. Crump (8061005) |
author_facet |
Kristina D. Baker (4020629) Colleen T. E. Kellogg (8060999) James W. McClelland (2909585) Kenneth H. Dunton (8061002) Byron C. Crump (8061005) |
author_sort |
Kristina D. Baker (4020629) |
title |
Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
title_short |
Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
title_full |
Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
title_fullStr |
Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
title_full_unstemmed |
Data_Sheet_1_The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.zip |
title_sort |
data_sheet_1_the genomic capabilities of microbial communities track seasonal variation in environmental conditions of arctic lagoons.zip |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.601901.s001 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Beaufort Sea Phytoplankton Alaska |
genre_facet |
Arctic Beaufort Sea Phytoplankton Alaska |
op_relation |
https://figshare.com/articles/dataset/Data_Sheet_1_The_Genomic_Capabilities_of_Microbial_Communities_Track_Seasonal_Variation_in_Environmental_Conditions_of_Arctic_Lagoons_zip/13939019 doi:10.3389/fmicb.2021.601901.s001 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.601901.s001 |
_version_ |
1766324478434869248 |