Supplementary Figure 2: Antibodies validated for routinely processed tissue unpredictably stain frozen tissue sections

• Main Authors: Giorgio Cattoretti (568042), Maddalena Bolognesi (10110697), Francesco Mascadri (10110700), Mario Faretta (429355), Francesca Bosisio (10110703)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Immunology not elsewhere classified; Antibodies; Immunolabeling; Validation; Aldrich; Triton; Orca
• Online Access: https://doi.org/10.25402/btn.13730758.v1

Supplementary figure 2 Effect of extended crosslinking on antibody penetration in single cultured cells (MCF10A, non-transformed immortalized breast epithelium). Cells were immunostained for detection of 53BP1 nuclear protein. A minor fraction of the protein is also present in the cytoplasm. Supplem...