A Bi-Enzymatic Cascade Pathway Towards FAHFAs
Recently discovered endogenous mammalian lipids fatty acid esters of hydroxy fatty acids (FAHFAs), proved to have anti-inflammatory and anti-diabetic effects . Due to their extremely low abundancies in vivo , forging a feasible scenario for FAHFA synthesis is critical for their use in uncovering bio...
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ftsmithonian:oai:figshare.com:article/13650629 2023-05-15T13:59:57+02:00 A Bi-Enzymatic Cascade Pathway Towards FAHFAs Yan Zhang (10031027) Bekir Engin Eser (6724040) Zheng Guo (6789574) 2021-01-28T12:48:26Z https://doi.org/10.26434/chemrxiv.13650629.v1 unknown https://figshare.com/articles/preprint/A_Bi-Enzymatic_Cascade_Pathway_Towards_FAHFAs/13650629 doi:10.26434/chemrxiv.13650629.v1 CC BY-NC-ND 4.0 CC-BY-NC-ND Biocatalysis bioactive FAHFAs enzymatic synthesis fatty acid hydratase lipase renewable biomass Text Preprint 2021 ftsmithonian https://doi.org/10.26434/chemrxiv.13650629.v1 2021-02-03T08:52:19Z Recently discovered endogenous mammalian lipids fatty acid esters of hydroxy fatty acids (FAHFAs), proved to have anti-inflammatory and anti-diabetic effects . Due to their extremely low abundancies in vivo , forging a feasible scenario for FAHFA synthesis is critical for their use in uncovering biological mechanism or clinical trials. Here, we showcase a fully enzymatic approach, a novel in vitro bi-enzymatic cascade system, enabling an effective conversion of nature-abundant fatty acid into FAHFAs. Two hydratases from L. acidophilus were used for converting unsaturated fatty acids to various stereospecific hydroxy fatty acids, followed by esterification with another fatty acid catalyzed by C. antarctica lipase A (CALA). Various FAHFAs were synthesized in a preparative scale using this bi-enzymatic approach in a one-pot two-step operation mode. In all, we demonstrated that hydratase-CALA system promises a sustainable solution to the synthesis of structure-diverse stereospecific FAHFAs. Report Antarc* Antarctica Unknown |
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Open Polar |
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op_collection_id |
ftsmithonian |
language |
unknown |
topic |
Biocatalysis bioactive FAHFAs enzymatic synthesis fatty acid hydratase lipase renewable biomass |
spellingShingle |
Biocatalysis bioactive FAHFAs enzymatic synthesis fatty acid hydratase lipase renewable biomass Yan Zhang (10031027) Bekir Engin Eser (6724040) Zheng Guo (6789574) A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
topic_facet |
Biocatalysis bioactive FAHFAs enzymatic synthesis fatty acid hydratase lipase renewable biomass |
description |
Recently discovered endogenous mammalian lipids fatty acid esters of hydroxy fatty acids (FAHFAs), proved to have anti-inflammatory and anti-diabetic effects . Due to their extremely low abundancies in vivo , forging a feasible scenario for FAHFA synthesis is critical for their use in uncovering biological mechanism or clinical trials. Here, we showcase a fully enzymatic approach, a novel in vitro bi-enzymatic cascade system, enabling an effective conversion of nature-abundant fatty acid into FAHFAs. Two hydratases from L. acidophilus were used for converting unsaturated fatty acids to various stereospecific hydroxy fatty acids, followed by esterification with another fatty acid catalyzed by C. antarctica lipase A (CALA). Various FAHFAs were synthesized in a preparative scale using this bi-enzymatic approach in a one-pot two-step operation mode. In all, we demonstrated that hydratase-CALA system promises a sustainable solution to the synthesis of structure-diverse stereospecific FAHFAs. |
format |
Report |
author |
Yan Zhang (10031027) Bekir Engin Eser (6724040) Zheng Guo (6789574) |
author_facet |
Yan Zhang (10031027) Bekir Engin Eser (6724040) Zheng Guo (6789574) |
author_sort |
Yan Zhang (10031027) |
title |
A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
title_short |
A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
title_full |
A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
title_fullStr |
A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
title_full_unstemmed |
A Bi-Enzymatic Cascade Pathway Towards FAHFAs |
title_sort |
bi-enzymatic cascade pathway towards fahfas |
publishDate |
2021 |
url |
https://doi.org/10.26434/chemrxiv.13650629.v1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://figshare.com/articles/preprint/A_Bi-Enzymatic_Cascade_Pathway_Towards_FAHFAs/13650629 doi:10.26434/chemrxiv.13650629.v1 |
op_rights |
CC BY-NC-ND 4.0 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.26434/chemrxiv.13650629.v1 |
_version_ |
1766268870608289792 |