Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield
Biofilm formation is a common adaptation for microbes in energy-limited conditions such as those prevalent in the vast deep terrestrial biosphere. However, due to the low biomass and the inaccessible nature of subsurface groundwaters, the microbial populations and genes involved in its formation are...
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ftslunivuppsala:oai:pub.epsilon.slu.se:31363 2023-08-27T04:09:23+02:00 Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield Lopez-Fernandez, Margarita Westmeijer, George Turner, Stephanie Broman, Elias Stahle, Magnus Bertilsson, Stefan Dopson, Mark 2023 application/pdf https://pub.epsilon.slu.se/31363/ https://pub.epsilon.slu.se/31363/1/lopez-fernandez-m-et-al-20230727.pdf en eng eng https://pub.epsilon.slu.se/31363/1/lopez-fernandez-m-et-al-20230727.pdf Lopez-Fernandez, Margarita and Westmeijer, George and Turner, Stephanie and Broman, Elias and Stahle, Magnus and Bertilsson, Stefan and Dopson, Mark (2023). Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield. npj Biofilms and Microbiomes. 9 :1 , 41 [Research article] Microbiology (Microbiology in the medical area to be 30109) Research article NonPeerReviewed 2023 ftslunivuppsala 2023-08-03T16:13:54Z Biofilm formation is a common adaptation for microbes in energy-limited conditions such as those prevalent in the vast deep terrestrial biosphere. However, due to the low biomass and the inaccessible nature of subsurface groundwaters, the microbial populations and genes involved in its formation are understudied. Here, a flow-cell system was designed to investigate biofilm formation under in situ conditions in two groundwaters of contrasting age and geochemistry at the aspo Hard Rock Laboratory, Sweden. Metatranscriptomes showed Thiobacillus, Sideroxydans, and Desulforegula to be abundant and together accounted for 31% of the transcripts in the biofilm communities. Differential expression analysis highlighted Thiobacillus to have a principal role in biofilm formation in these oligotrophic groundwaters by being involved in relevant processes such as the formation of extracellular matrix, quorum sensing, and cell motility. The findings revealed an active biofilm community with sulfur cycling as a prominent mode of energy conservation in the deep biosphere. Article in Journal/Newspaper Fennoscandian Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
institution |
Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Microbiology (Microbiology in the medical area to be 30109) |
spellingShingle |
Microbiology (Microbiology in the medical area to be 30109) Lopez-Fernandez, Margarita Westmeijer, George Turner, Stephanie Broman, Elias Stahle, Magnus Bertilsson, Stefan Dopson, Mark Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
topic_facet |
Microbiology (Microbiology in the medical area to be 30109) |
description |
Biofilm formation is a common adaptation for microbes in energy-limited conditions such as those prevalent in the vast deep terrestrial biosphere. However, due to the low biomass and the inaccessible nature of subsurface groundwaters, the microbial populations and genes involved in its formation are understudied. Here, a flow-cell system was designed to investigate biofilm formation under in situ conditions in two groundwaters of contrasting age and geochemistry at the aspo Hard Rock Laboratory, Sweden. Metatranscriptomes showed Thiobacillus, Sideroxydans, and Desulforegula to be abundant and together accounted for 31% of the transcripts in the biofilm communities. Differential expression analysis highlighted Thiobacillus to have a principal role in biofilm formation in these oligotrophic groundwaters by being involved in relevant processes such as the formation of extracellular matrix, quorum sensing, and cell motility. The findings revealed an active biofilm community with sulfur cycling as a prominent mode of energy conservation in the deep biosphere. |
format |
Article in Journal/Newspaper |
author |
Lopez-Fernandez, Margarita Westmeijer, George Turner, Stephanie Broman, Elias Stahle, Magnus Bertilsson, Stefan Dopson, Mark |
author_facet |
Lopez-Fernandez, Margarita Westmeijer, George Turner, Stephanie Broman, Elias Stahle, Magnus Bertilsson, Stefan Dopson, Mark |
author_sort |
Lopez-Fernandez, Margarita |
title |
Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
title_short |
Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
title_full |
Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
title_fullStr |
Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
title_full_unstemmed |
Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield |
title_sort |
thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the fennoscandian shield |
publishDate |
2023 |
url |
https://pub.epsilon.slu.se/31363/ https://pub.epsilon.slu.se/31363/1/lopez-fernandez-m-et-al-20230727.pdf |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_relation |
https://pub.epsilon.slu.se/31363/1/lopez-fernandez-m-et-al-20230727.pdf Lopez-Fernandez, Margarita and Westmeijer, George and Turner, Stephanie and Broman, Elias and Stahle, Magnus and Bertilsson, Stefan and Dopson, Mark (2023). Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield. npj Biofilms and Microbiomes. 9 :1 , 41 [Research article] |
_version_ |
1775350602714316800 |