Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding
Arctic charr (Salvelinus alpinus) is a high-value species for the Nordic aquaculture. The highly variable reproductive performance that is commonly observed in commercial farms is hindering the expansion of the Arctic charr industry in Sweden. Traits related to sperm motility (total motility; curvil...
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ftslunivuppsala:oai:pub.epsilon.slu.se:29319 2023-05-15T14:25:18+02:00 Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding Kurta, Khrystyna Jeuthe, Henrik De Koning, Dirk-Jan Palaiokostas, Christos 2022 application/pdf https://pub.epsilon.slu.se/29319/ https://pub.epsilon.slu.se/29319/1/kurta-k-et-al-20221019.pdf en eng eng https://pub.epsilon.slu.se/29319/1/kurta-k-et-al-20221019.pdf Kurta, Khrystyna and Jeuthe, Henrik and De Koning, Dirk-Jan and Palaiokostas, Christos (2022). Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding. Aquaculture Reports. 25 , 101234 [Research article] Fish and Aquacultural Science Research article NonPeerReviewed info:eu-repo/semantics/article 2022 ftslunivuppsala 2022-10-20T16:13:51Z Arctic charr (Salvelinus alpinus) is a high-value species for the Nordic aquaculture. The highly variable reproductive performance that is commonly observed in commercial farms is hindering the expansion of the Arctic charr industry in Sweden. Traits related to sperm motility (total motility; curvilinear velocity; average path velocity; straight-line velocity) and concentration can play a pivotal role in male fertility. Selective breeding practices could offer solutions and contribute to improving male fertility. The current study aimed to investigate the magnitude of genetic variance for sperm quality traits in a selectively bred population of Arctic charr from Sweden and evaluate the possibility of their improvement through selection. Sperm motility and concentration were recorded using a computer-assisted semen analysis (CASA) system and a NucleoCounter, respectively, in over 400 males from year-class 2017. Double digest restriction-site associated DNA sequencing (ddRAD-seq) was applied in a subset of the recorded animals (n = 329), resulting in the detection of over 5000 single nucleotide polymorphisms (SNPs). Moderate heritability estimates were obtained for the recorded semen traits using both pedigree (0.21-0.32; SE 0.09) and genomic (0.23-0.26; SE 0.09) relationship matrices. A genome-wide association study (GWAS) detected a single SNP significantly associated (P < 1e-05) with total sperm motility on chromosome LG7 in relatively close proximity (500 Kb) to PTPN11 a gene previously associated with sperm quality traits in mammals. Moreover, weighted single-step genomic best linear unbiased prediction (WssGBLUP) pinpointed genomic regions explaining more than 3 % of the additive genetic variance for both the motility traits and the sperm concentration. Finally, the efficiency of genomic prediction was tested using a 3-fold cross-validation scheme. Higher prediction accuracy for total motility and velocities (both curvilinear and average path) was obtained using genomic information (0.26-0.29, SE ... Article in Journal/Newspaper Arctic Arctic charr Arctic Salvelinus alpinus Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive Arctic |
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Open Polar |
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Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Fish and Aquacultural Science |
spellingShingle |
Fish and Aquacultural Science Kurta, Khrystyna Jeuthe, Henrik De Koning, Dirk-Jan Palaiokostas, Christos Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
topic_facet |
Fish and Aquacultural Science |
description |
Arctic charr (Salvelinus alpinus) is a high-value species for the Nordic aquaculture. The highly variable reproductive performance that is commonly observed in commercial farms is hindering the expansion of the Arctic charr industry in Sweden. Traits related to sperm motility (total motility; curvilinear velocity; average path velocity; straight-line velocity) and concentration can play a pivotal role in male fertility. Selective breeding practices could offer solutions and contribute to improving male fertility. The current study aimed to investigate the magnitude of genetic variance for sperm quality traits in a selectively bred population of Arctic charr from Sweden and evaluate the possibility of their improvement through selection. Sperm motility and concentration were recorded using a computer-assisted semen analysis (CASA) system and a NucleoCounter, respectively, in over 400 males from year-class 2017. Double digest restriction-site associated DNA sequencing (ddRAD-seq) was applied in a subset of the recorded animals (n = 329), resulting in the detection of over 5000 single nucleotide polymorphisms (SNPs). Moderate heritability estimates were obtained for the recorded semen traits using both pedigree (0.21-0.32; SE 0.09) and genomic (0.23-0.26; SE 0.09) relationship matrices. A genome-wide association study (GWAS) detected a single SNP significantly associated (P < 1e-05) with total sperm motility on chromosome LG7 in relatively close proximity (500 Kb) to PTPN11 a gene previously associated with sperm quality traits in mammals. Moreover, weighted single-step genomic best linear unbiased prediction (WssGBLUP) pinpointed genomic regions explaining more than 3 % of the additive genetic variance for both the motility traits and the sperm concentration. Finally, the efficiency of genomic prediction was tested using a 3-fold cross-validation scheme. Higher prediction accuracy for total motility and velocities (both curvilinear and average path) was obtained using genomic information (0.26-0.29, SE ... |
format |
Article in Journal/Newspaper |
author |
Kurta, Khrystyna Jeuthe, Henrik De Koning, Dirk-Jan Palaiokostas, Christos |
author_facet |
Kurta, Khrystyna Jeuthe, Henrik De Koning, Dirk-Jan Palaiokostas, Christos |
author_sort |
Kurta, Khrystyna |
title |
Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
title_short |
Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
title_full |
Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
title_fullStr |
Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
title_full_unstemmed |
Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding |
title_sort |
evaluating the potential of improving sperm quality traits in farmed arctic charr (salvelinus alpinus) using selective breeding |
publishDate |
2022 |
url |
https://pub.epsilon.slu.se/29319/ https://pub.epsilon.slu.se/29319/1/kurta-k-et-al-20221019.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic Arctic charr Arctic Salvelinus alpinus |
op_relation |
https://pub.epsilon.slu.se/29319/1/kurta-k-et-al-20221019.pdf Kurta, Khrystyna and Jeuthe, Henrik and De Koning, Dirk-Jan and Palaiokostas, Christos (2022). Evaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding. Aquaculture Reports. 25 , 101234 [Research article] |
_version_ |
1766297733543493632 |