Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stabl...
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ftslunivuppsala:oai:pub.epsilon.slu.se:28448 2023-05-15T17:45:10+02:00 Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems Magh, Ruth Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kubert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John 2022 application/pdf https://pub.epsilon.slu.se/28448/ https://pub.epsilon.slu.se/28448/1/magh-r-k-et-al-220816.pdf en eng eng https://pub.epsilon.slu.se/28448/1/magh-r-k-et-al-220816.pdf Magh, Ruth and Magh, Ruth-Kristina and Gralher, Benjamin and Herbstritt, Barbara and Kubert, Angelika and Lim, Hyungwoo and Lundmark, Tomas and Marshall, John (2022). Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems. Hydrology and Earth System Sciences. 26 :13 , 3573-3587 [Research article] Oceanography Hydrology Water Resources Geosciences Multidisciplinary Research article NonPeerReviewed info:eu-repo/semantics/article 2022 ftslunivuppsala 2022-10-13T16:13:51Z Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from -95 parts per thousand to +1700 parts per thousand for delta H-2) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 parts per thousand to 4.4 parts per thousand for delta H-2 and 0.6 parts per thousand to 0.8 parts per thousand for delta O-18 for natural-abundance samples. Although H-2-enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for delta O-18 but observed higher variation in delta H-2 than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring ... Article in Journal/Newspaper Northern Sweden Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
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Open Polar |
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Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
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ftslunivuppsala |
language |
English |
topic |
Oceanography Hydrology Water Resources Geosciences Multidisciplinary |
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Oceanography Hydrology Water Resources Geosciences Multidisciplinary Magh, Ruth Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kubert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
topic_facet |
Oceanography Hydrology Water Resources Geosciences Multidisciplinary |
description |
Using water-stable isotopes to track plant water uptake or soil water processes has become an invaluable tool in ecohydrology and physiological ecology. Recent studies have shown that laser absorption spectroscopy can measure equilibrated water vapour well enough to support inference of liquid-stable isotope composition of plant or soil water, on-site and in real-time. However, current in situ systems require the presence of an instrument in the field. Here we tested, first in the lab and then in the field, a method for equilibrating, collecting, storing, and finally analysing water vapour for its isotopic composition that does not require an instrument in the field. We developed a vapour storage vial system (VSVS) that relies on in situ sampling into crimp neck vials with a double-coated cap using a pump and a flow metre powered through a small battery and measuring the samples in a laboratory. All components are inexpensive and commercially available. We tested the system's ability to store the isotopic composition of its contents by sampling a range of water vapour of known isotopic compositions (from -95 parts per thousand to +1700 parts per thousand for delta H-2) and measuring the isotopic composition after different storage periods. Samples for the field trial were taken in a boreal forest in northern Sweden. The isotopic composition was maintained to within 0.6 parts per thousand to 4.4 parts per thousand for delta H-2 and 0.6 parts per thousand to 0.8 parts per thousand for delta O-18 for natural-abundance samples. Although H-2-enriched samples showed greater uncertainty, they were sufficient to quantify label amounts. We detected a small change in the isotopic composition of the sample after a long storage period, but it was correctable by linear regression models. We observed the same trend for the samples obtained in the field trial for delta O-18 but observed higher variation in delta H-2 than in the lab trial. Our method combines the best of two worlds, sampling many trees in situ while measuring ... |
format |
Article in Journal/Newspaper |
author |
Magh, Ruth Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kubert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John |
author_facet |
Magh, Ruth Magh, Ruth-Kristina Gralher, Benjamin Herbstritt, Barbara Kubert, Angelika Lim, Hyungwoo Lundmark, Tomas Marshall, John |
author_sort |
Magh, Ruth |
title |
Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_short |
Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_full |
Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_fullStr |
Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_full_unstemmed |
Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
title_sort |
technical note: conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems |
publishDate |
2022 |
url |
https://pub.epsilon.slu.se/28448/ https://pub.epsilon.slu.se/28448/1/magh-r-k-et-al-220816.pdf |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
https://pub.epsilon.slu.se/28448/1/magh-r-k-et-al-220816.pdf Magh, Ruth and Magh, Ruth-Kristina and Gralher, Benjamin and Herbstritt, Barbara and Kubert, Angelika and Lim, Hyungwoo and Lundmark, Tomas and Marshall, John (2022). Technical note: Conservative storage of water vapour - practical in situ sampling of stable isotopes in tree stems. Hydrology and Earth System Sciences. 26 :13 , 3573-3587 [Research article] |
_version_ |
1766147974719602688 |