DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus)
Despite the relatively long history of captive breeding, the Arctic charr still exhibits a generally low, but highly variable reproductive performance in aquaculture. A recent publication exposed potential paternal factors influencing the reproductive outcome of Arctic charr broodstock from the Swed...
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ftslunivuppsala:oai:pub.epsilon.slu.se:26600 2023-05-15T14:23:49+02:00 DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) Jeuthe, Henrik Palaiokostas, Christos Johannisson, Anders 2022 application/pdf https://pub.epsilon.slu.se/26600/ https://pub.epsilon.slu.se/26600/1/jeuthe_h_et_al_211227.pdf en eng eng https://pub.epsilon.slu.se/26600/1/jeuthe_h_et_al_211227.pdf Jeuthe, Henrik and Palaiokostas, Christos and Johannisson, Anders (2022). DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus). Aquaculture. 547 , 737537 [Research article] Fish and Aquacultural Science Research article NonPeerReviewed info:eu-repo/semantics/article 2022 ftslunivuppsala 2022-01-09T19:16:59Z Despite the relatively long history of captive breeding, the Arctic charr still exhibits a generally low, but highly variable reproductive performance in aquaculture. A recent publication exposed potential paternal factors influencing the reproductive outcome of Arctic charr broodstock from the Swedish breeding program. Interestingly, the paternal factor appeared to be more closely connected to embryo survival than to fertilisation rates. This lead to speculations on whether e.g. chromatin related issues, potentially related to oxidative stress could be involved. In order to investigate this hypothesis the present study assessed the levels of DNA fragmentation, using the SCD-method, and membrane integrity, using flow cytometry, in sperm of farmed Arctic charr. Moreover, the existence of associations was tested between DNA fragmentation and membrane integrity in individual semen samples and viability of their resulting progeny. We found high levels of DNA fragmentation in sperm from the Arctic charr sires, ranging from 24% to 86% with a median of 67%. Membrane integrity values were high, with individual levels of 93.1% to 99.6% viable sperm cells, median 98.8%. DNA fragmentation and membrane integrity values were moderately correlated (r = 0.304, p < 0.05). Fertilisation rates and proportions of eyed eggs showed substantial individual variation and were correlated (r = 0.497, p < 0.05). However, large differences between proportion of eyed eggs and fertilisation rate, median 52% and 81.6% respectively, highlight that the main loss occurred due to embryo mortality rather than failed fertilisation. No correlation was found between either DNA fragmentation or membrane integrity and the resulting reproductive outcome (fertilisation rate and eyed eggs) of the individual Arctic charr sires. Overall, our study identified very high levels of DNA fragmentation, which could influence the fertility of the broodstock in question and thereby be a mitigating mechanism involved in the low reproductive success most often observed in farmed Arctic charr. Further exploration of this relationship would be needed, though. Article in Journal/Newspaper Arctic Arctic charr Arctic Salvelinus alpinus Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive Arctic |
institution |
Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Fish and Aquacultural Science |
spellingShingle |
Fish and Aquacultural Science Jeuthe, Henrik Palaiokostas, Christos Johannisson, Anders DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
topic_facet |
Fish and Aquacultural Science |
description |
Despite the relatively long history of captive breeding, the Arctic charr still exhibits a generally low, but highly variable reproductive performance in aquaculture. A recent publication exposed potential paternal factors influencing the reproductive outcome of Arctic charr broodstock from the Swedish breeding program. Interestingly, the paternal factor appeared to be more closely connected to embryo survival than to fertilisation rates. This lead to speculations on whether e.g. chromatin related issues, potentially related to oxidative stress could be involved. In order to investigate this hypothesis the present study assessed the levels of DNA fragmentation, using the SCD-method, and membrane integrity, using flow cytometry, in sperm of farmed Arctic charr. Moreover, the existence of associations was tested between DNA fragmentation and membrane integrity in individual semen samples and viability of their resulting progeny. We found high levels of DNA fragmentation in sperm from the Arctic charr sires, ranging from 24% to 86% with a median of 67%. Membrane integrity values were high, with individual levels of 93.1% to 99.6% viable sperm cells, median 98.8%. DNA fragmentation and membrane integrity values were moderately correlated (r = 0.304, p < 0.05). Fertilisation rates and proportions of eyed eggs showed substantial individual variation and were correlated (r = 0.497, p < 0.05). However, large differences between proportion of eyed eggs and fertilisation rate, median 52% and 81.6% respectively, highlight that the main loss occurred due to embryo mortality rather than failed fertilisation. No correlation was found between either DNA fragmentation or membrane integrity and the resulting reproductive outcome (fertilisation rate and eyed eggs) of the individual Arctic charr sires. Overall, our study identified very high levels of DNA fragmentation, which could influence the fertility of the broodstock in question and thereby be a mitigating mechanism involved in the low reproductive success most often observed in farmed Arctic charr. Further exploration of this relationship would be needed, though. |
format |
Article in Journal/Newspaper |
author |
Jeuthe, Henrik Palaiokostas, Christos Johannisson, Anders |
author_facet |
Jeuthe, Henrik Palaiokostas, Christos Johannisson, Anders |
author_sort |
Jeuthe, Henrik |
title |
DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
title_short |
DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
title_full |
DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
title_fullStr |
DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
title_full_unstemmed |
DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus) |
title_sort |
dna fragmentation and membrane integrity in sperm of farmed arctic charr (salvelinus alpinus) |
publishDate |
2022 |
url |
https://pub.epsilon.slu.se/26600/ https://pub.epsilon.slu.se/26600/1/jeuthe_h_et_al_211227.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic Arctic charr Arctic Salvelinus alpinus |
op_relation |
https://pub.epsilon.slu.se/26600/1/jeuthe_h_et_al_211227.pdf Jeuthe, Henrik and Palaiokostas, Christos and Johannisson, Anders (2022). DNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus). Aquaculture. 547 , 737537 [Research article] |
_version_ |
1766296302724841472 |