Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes
Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50 degrees North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the divers...
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ftslunivuppsala:oai:pub.epsilon.slu.se:25305 2023-05-15T18:28:39+02:00 Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes Martin, Gaëtan Rissanen, Antti J. Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Peura, Sari 2021 application/pdf https://pub.epsilon.slu.se/25305/ https://pub.epsilon.slu.se/25305/1/martin_g_et_al_210910.pdf en eng eng https://pub.epsilon.slu.se/25305/1/martin_g_et_al_210910.pdf Martin, Gaëtan and Rissanen, Antti J. and Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Peura, Sari (2021). Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes. Frontiers in Microbiology. 12 , 669937 [Research article] Microbiology (Microbiology in the medical area to be 30109) Research article NonPeerReviewed info:eu-repo/semantics/article 2021 ftslunivuppsala 2022-01-09T19:16:40Z Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50 degrees North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the diversity and distribution of the methanotrophs in lakes are still poorly understood. Here, we used shotgun metagenomic data to explore the diversity and distribution of methanotrophs in 40 oxygen-stratified water bodies in boreal and subarctic areas in Europe and North America. In our data, gammaproteobacterial methanotrophs (order Methylococcales) generally dominated the methanotrophic communities throughout the water columns. A recently discovered lineage of Methylococcales, Candidatus Methylumidiphilus, was present in all the studied water bodies and dominated the methanotrophic community in lakes with a high relative abundance of methanotrophs. Alphaproteobacterial methanotrophs were the second most abundant group of methanotrophs. In the top layer of the lakes, characterized by low CH4 concentration, their abundance could surpass that of the gammaproteobacterial methanotrophs. These results support the theory that the alphaproteobacterial methanotrophs have a high affinity for CH4 and can be considered stress-tolerant strategists. In contrast, the gammaproteobacterial methanotrophs are competitive strategists. In addition, relative abundances of anaerobic methanotrophs, Candidatus Methanoperedenaceae and Candidatus Methylomirabilis, were strongly correlated, suggesting possible co-metabolism. Our data also suggest that these anaerobic methanotrophs could be active even in the oxic layers. In non-metric multidimensional scaling, alpha- and gammaproteobacterial methanotrophs formed separate clusters based on their abundances in the samples, except for the gammaproteobacterial Candidatus Methylumidiphilus, which was separated from these two clusters. This may reflect similarities in the niche and environmental requirements of the different genera within alpha- and gammaproteobacterial methanotrophs. Our study confirms the importance of O-2 and CH4 in shaping the methanotrophic communities and suggests that one variable cannot explain the diversity and distribution of the methanotrophs across lakes. Instead, we suggest that the diversity and distribution of freshwater methanotrophs are regulated by lake-specific factors. Article in Journal/Newspaper Subarctic Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
institution |
Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Microbiology (Microbiology in the medical area to be 30109) |
spellingShingle |
Microbiology (Microbiology in the medical area to be 30109) Martin, Gaëtan Rissanen, Antti J. Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Peura, Sari Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
topic_facet |
Microbiology (Microbiology in the medical area to be 30109) |
description |
Boreal lakes and ponds produce two-thirds of the total natural methane emissions above the latitude of 50 degrees North. These lake emissions are regulated by methanotrophs which can oxidize up to 99% of the methane produced in the sediments and the water column. Despite their importance, the diversity and distribution of the methanotrophs in lakes are still poorly understood. Here, we used shotgun metagenomic data to explore the diversity and distribution of methanotrophs in 40 oxygen-stratified water bodies in boreal and subarctic areas in Europe and North America. In our data, gammaproteobacterial methanotrophs (order Methylococcales) generally dominated the methanotrophic communities throughout the water columns. A recently discovered lineage of Methylococcales, Candidatus Methylumidiphilus, was present in all the studied water bodies and dominated the methanotrophic community in lakes with a high relative abundance of methanotrophs. Alphaproteobacterial methanotrophs were the second most abundant group of methanotrophs. In the top layer of the lakes, characterized by low CH4 concentration, their abundance could surpass that of the gammaproteobacterial methanotrophs. These results support the theory that the alphaproteobacterial methanotrophs have a high affinity for CH4 and can be considered stress-tolerant strategists. In contrast, the gammaproteobacterial methanotrophs are competitive strategists. In addition, relative abundances of anaerobic methanotrophs, Candidatus Methanoperedenaceae and Candidatus Methylomirabilis, were strongly correlated, suggesting possible co-metabolism. Our data also suggest that these anaerobic methanotrophs could be active even in the oxic layers. In non-metric multidimensional scaling, alpha- and gammaproteobacterial methanotrophs formed separate clusters based on their abundances in the samples, except for the gammaproteobacterial Candidatus Methylumidiphilus, which was separated from these two clusters. This may reflect similarities in the niche and environmental requirements of the different genera within alpha- and gammaproteobacterial methanotrophs. Our study confirms the importance of O-2 and CH4 in shaping the methanotrophic communities and suggests that one variable cannot explain the diversity and distribution of the methanotrophs across lakes. Instead, we suggest that the diversity and distribution of freshwater methanotrophs are regulated by lake-specific factors. |
format |
Article in Journal/Newspaper |
author |
Martin, Gaëtan Rissanen, Antti J. Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Peura, Sari |
author_facet |
Martin, Gaëtan Rissanen, Antti J. Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Peura, Sari |
author_sort |
Martin, Gaëtan |
title |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_short |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_full |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_fullStr |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_full_unstemmed |
Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes |
title_sort |
candidatus methylumidiphilus drives peaks in methanotrophic relative abundance in stratified lakes and ponds across northern landscapes |
publishDate |
2021 |
url |
https://pub.epsilon.slu.se/25305/ https://pub.epsilon.slu.se/25305/1/martin_g_et_al_210910.pdf |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
https://pub.epsilon.slu.se/25305/1/martin_g_et_al_210910.pdf Martin, Gaëtan and Rissanen, Antti J. and Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Peura, Sari (2021). Candidatus Methylumidiphilus Drives Peaks in Methanotrophic Relative Abundance in Stratified Lakes and Ponds Across Northern Landscapes. Frontiers in Microbiology. 12 , 669937 [Research article] |
_version_ |
1766211201381957632 |