Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction
Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury....
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ftslunivuppsala:oai:pub.epsilon.slu.se:25179 2023-05-15T18:42:11+02:00 Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction Givre, Lucas Da Silva, Claire Crola Swenson, Jon E. Arnemo, Jon Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic 2021 application/pdf https://pub.epsilon.slu.se/25179/ https://pub.epsilon.slu.se/25179/1/givre_l_et_al_210903.pdf en eng eng https://pub.epsilon.slu.se/25179/1/givre_l_et_al_210903.pdf Givre, Lucas and Da Silva, Claire Crola and Swenson, Jon E. and Arnemo, Jon and Gauquelin-Koch, Guillemette and Bertile, Fabrice and Lefai, Etienne and Gomez, Ludovic (2021). Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction. Frontiers in Cardiovascular Medicine. 8 , 687501 [Research article] Clinical Science Research article NonPeerReviewed 2021 ftslunivuppsala 2022-01-09T19:16:37Z Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury. Interestingly, despite physical inactivity during hibernation, brown bears (Ursus arctos) cope with cardiovascular physiological conditions that would be detrimental to humans. We hypothesized that bear serum might contain circulating factors that could provide protection against cell injury. In this study, we sought to determine whether addition of bear serum might improve cardiomyocyte survival following hypoxia-reoxygenation. Isolated mouse cardiomyocytes underwent 45 min of hypoxia followed by reoxygenation. At the onset of reoxygenation, cells received fetal bovine serum (FBS; positive control), summer (SBS) or winter bear serum (WBS), or adult serums of other species, as indicated. After 2 h of reoxygenation, propidium iodide staining was used to evaluate cell viability by flow cytometry. Whereas, 0.5% SBS tended to decrease reperfusion injury, 0.5% WBS significantly reduced cell death, averaging 74.04 +/- 7.06% vs. 79.20 +/- 6.53% in the FBS group. This cardioprotective effect was lost at 0.1%, became toxic above 5%, and was specific to the bear. Our results showed that bear serum exerts a therapeutic effect with an efficacy threshold, an optimal dose, and a toxic effect on cardiomyocyte viability after hypoxia-reoxygenation. Therefore, the bear serum may be a potential source for identifying new therapeutic molecules to fight against myocardial reperfusion injury and cell death in general. Article in Journal/Newspaper Ursus arctos Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
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Open Polar |
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Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Clinical Science |
spellingShingle |
Clinical Science Givre, Lucas Da Silva, Claire Crola Swenson, Jon E. Arnemo, Jon Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
topic_facet |
Clinical Science |
description |
Ischemic heart disease remains one of the leading causes of death worldwide. Despite intensive research on the treatment of acute myocardial infarction, no effective therapy has shown clinical success. Therefore, novel therapeutic strategies are required to protect the heart from reperfusion injury. Interestingly, despite physical inactivity during hibernation, brown bears (Ursus arctos) cope with cardiovascular physiological conditions that would be detrimental to humans. We hypothesized that bear serum might contain circulating factors that could provide protection against cell injury. In this study, we sought to determine whether addition of bear serum might improve cardiomyocyte survival following hypoxia-reoxygenation. Isolated mouse cardiomyocytes underwent 45 min of hypoxia followed by reoxygenation. At the onset of reoxygenation, cells received fetal bovine serum (FBS; positive control), summer (SBS) or winter bear serum (WBS), or adult serums of other species, as indicated. After 2 h of reoxygenation, propidium iodide staining was used to evaluate cell viability by flow cytometry. Whereas, 0.5% SBS tended to decrease reperfusion injury, 0.5% WBS significantly reduced cell death, averaging 74.04 +/- 7.06% vs. 79.20 +/- 6.53% in the FBS group. This cardioprotective effect was lost at 0.1%, became toxic above 5%, and was specific to the bear. Our results showed that bear serum exerts a therapeutic effect with an efficacy threshold, an optimal dose, and a toxic effect on cardiomyocyte viability after hypoxia-reoxygenation. Therefore, the bear serum may be a potential source for identifying new therapeutic molecules to fight against myocardial reperfusion injury and cell death in general. |
format |
Article in Journal/Newspaper |
author |
Givre, Lucas Da Silva, Claire Crola Swenson, Jon E. Arnemo, Jon Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic |
author_facet |
Givre, Lucas Da Silva, Claire Crola Swenson, Jon E. Arnemo, Jon Gauquelin-Koch, Guillemette Bertile, Fabrice Lefai, Etienne Gomez, Ludovic |
author_sort |
Givre, Lucas |
title |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_short |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_full |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_fullStr |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_full_unstemmed |
Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction |
title_sort |
cardiomyocyte protection by hibernating brown bear serum: toward the identification of new protective molecules against myocardial infarction |
publishDate |
2021 |
url |
https://pub.epsilon.slu.se/25179/ https://pub.epsilon.slu.se/25179/1/givre_l_et_al_210903.pdf |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
https://pub.epsilon.slu.se/25179/1/givre_l_et_al_210903.pdf Givre, Lucas and Da Silva, Claire Crola and Swenson, Jon E. and Arnemo, Jon and Gauquelin-Koch, Guillemette and Bertile, Fabrice and Lefai, Etienne and Gomez, Ludovic (2021). Cardiomyocyte Protection by Hibernating Brown Bear Serum: Toward the Identification of New Protective Molecules Against Myocardial Infarction. Frontiers in Cardiovascular Medicine. 8 , 687501 [Research article] |
_version_ |
1766231808711589888 |