Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations
Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional eco...
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ftslunivuppsala:oai:pub.epsilon.slu.se:24885 2023-05-15T18:28:30+02:00 Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson M. Neufeld, Josh D. McMahon, Katherine D. Bertilsson, Stefan Greening, Christian Peura, Sari 2021 application/pdf https://pub.epsilon.slu.se/24885/ https://pub.epsilon.slu.se/24885/1/garcia_sl_et_al_210812.pdf en eng eng https://pub.epsilon.slu.se/24885/1/garcia_sl_et_al_210812.pdf Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Tsuji, Jackson M. and Neufeld, Josh D. and McMahon, Katherine D. and Bertilsson, Stefan and Greening, Christian and Peura, Sari (2021). Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations. mSystems. 6 , e01196-20 [Research article] Microbiology (Microbiology in the medical area to be 30109) Research article NonPeerReviewed info:eu-repo/semantics/article 2021 ftslunivuppsala 2022-09-15T16:13:55Z Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems. Article in Journal/Newspaper Subarctic Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
institution |
Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
topic |
Microbiology (Microbiology in the medical area to be 30109) |
spellingShingle |
Microbiology (Microbiology in the medical area to be 30109) Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson M. Neufeld, Josh D. McMahon, Katherine D. Bertilsson, Stefan Greening, Christian Peura, Sari Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
topic_facet |
Microbiology (Microbiology in the medical area to be 30109) |
description |
Photosynthetic bacteria from the class Chlorobia (formerly phylum Chlorobi) sustain carbon fixation in anoxic water columns. They harvest light at extremely low intensities and use various inorganic electron donors to fix carbon dioxide into biomass. Until now, most information on the functional ecology and local adaptations of Chlorobia members came from isolates and merely 26 sequenced genomes that may not adequately represent natural populations. To address these limitations, we analyzed global metagenomes to profile planktonic Chlorobia cells from the oxyclines of 42 freshwater bodies, spanning subarctic to tropical regions and encompassing all four seasons. We assembled and compiled over 500 genomes, including metagenome-assembled genomes (MAGs), single-amplified genomes (SAGs), and reference genomes from cultures, clustering them into 71 metagenomic operational taxonomic units (mOTUs or “species”). Of the 71 mOTUs, 57 were classified within the genus Chlorobium, and these mOTUs represented up to ∼60% of the microbial communities in the sampled anoxic waters. Several Chlorobium-associated mOTUs were globally distributed, whereas others were endemic to individual lakes. Although most clades encoded the ability to oxidize hydrogen, many lacked genes for the oxidation of specific sulfur and iron substrates. Surprisingly, one globally distributed Scandinavian clade encoded the ability to oxidize hydrogen, sulfur, and iron, suggesting that metabolic versatility facilitated such widespread colonization. Overall, these findings provide new insight into the biogeography of the Chlorobia and the metabolic traits that facilitate niche specialization within lake ecosystems. |
format |
Article in Journal/Newspaper |
author |
Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson M. Neufeld, Josh D. McMahon, Katherine D. Bertilsson, Stefan Greening, Christian Peura, Sari |
author_facet |
Garcia, Sarahi L. Mehrshad, Maliheh Buck, Moritz Tsuji, Jackson M. Neufeld, Josh D. McMahon, Katherine D. Bertilsson, Stefan Greening, Christian Peura, Sari |
author_sort |
Garcia, Sarahi L. |
title |
Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
title_short |
Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
title_full |
Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
title_fullStr |
Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
title_full_unstemmed |
Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
title_sort |
freshwater chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations |
publishDate |
2021 |
url |
https://pub.epsilon.slu.se/24885/ https://pub.epsilon.slu.se/24885/1/garcia_sl_et_al_210812.pdf |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
https://pub.epsilon.slu.se/24885/1/garcia_sl_et_al_210812.pdf Garcia, Sarahi L. and Mehrshad, Maliheh and Buck, Moritz and Tsuji, Jackson M. and Neufeld, Josh D. and McMahon, Katherine D. and Bertilsson, Stefan and Greening, Christian and Peura, Sari (2021). Freshwater Chlorobia exhibit metabolic specialization among cosmopolitan and endemic populations. mSystems. 6 , e01196-20 [Research article] |
_version_ |
1766211008603357184 |