Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique
Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, CrimeanCongo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present...
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ftslunivuppsala:oai:pub.epsilon.slu.se:15903 2023-05-15T15:55:57+02:00 Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando Chanisso Falk, Kerstin Fafetine, José Berg, Mikael Blomström, Anne-Lie 2018 application/pdf https://pub.epsilon.slu.se/15903/ https://pub.epsilon.slu.se/15903/1/cholleti_et_al_190212.pdf https://doi.org/10.1080/20008686.2018.1478585 sv eng swe eng https://pub.epsilon.slu.se/15903/1/cholleti_et_al_190212.pdf Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie (2018). Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique. Infection ecology & epidemiology. 8 , 1-8 [Research article] cc_by_4 CC-BY Pathobiology Public Health Global Health Social Medicine and Epidemiology Research article PeerReviewed 2018 ftslunivuppsala https://doi.org/10.1080/20008686.2018.1478585 2022-01-09T19:14:42Z Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, CrimeanCongo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus. Article in Journal/Newspaper Common Eider Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive Infection Ecology & Epidemiology 8 1 1478585 |
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Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
Swedish English |
topic |
Pathobiology Public Health Global Health Social Medicine and Epidemiology |
spellingShingle |
Pathobiology Public Health Global Health Social Medicine and Epidemiology Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando Chanisso Falk, Kerstin Fafetine, José Berg, Mikael Blomström, Anne-Lie Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
topic_facet |
Pathobiology Public Health Global Health Social Medicine and Epidemiology |
description |
Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, CrimeanCongo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus. |
format |
Article in Journal/Newspaper |
author |
Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando Chanisso Falk, Kerstin Fafetine, José Berg, Mikael Blomström, Anne-Lie |
author_facet |
Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando Chanisso Falk, Kerstin Fafetine, José Berg, Mikael Blomström, Anne-Lie |
author_sort |
Cholleti, Harindranath |
title |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_short |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_fullStr |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full_unstemmed |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_sort |
viral metagenomics reveals the presence of highly divergent quaranjavirus in rhipicephalus ticks from mozambique |
publishDate |
2018 |
url |
https://pub.epsilon.slu.se/15903/ https://pub.epsilon.slu.se/15903/1/cholleti_et_al_190212.pdf https://doi.org/10.1080/20008686.2018.1478585 |
genre |
Common Eider |
genre_facet |
Common Eider |
op_relation |
https://pub.epsilon.slu.se/15903/1/cholleti_et_al_190212.pdf Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie (2018). Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique. Infection ecology & epidemiology. 8 , 1-8 [Research article] |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1080/20008686.2018.1478585 |
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Infection Ecology & Epidemiology |
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8 |
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1 |
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1478585 |
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