Endocrine regulation of early sexual maturation in male Atlantic salmon parr
This thesis deals with changes in gene expression during activation of the brain-pituitary-gonadal axis at puberty in early maturing male Atlantic salmon (Salmo salar) parr. To help elucidate the physiological roles of gonadotropins and their receptors in the regulation of puberty, cDNAs encoding FS...
Main Author: | |
---|---|
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | https://pub.epsilon.slu.se/1331/ https://pub.epsilon.slu.se/1331/3/Summary_Maugars.pdf |
id |
ftslunivuppsala:oai:pub.epsilon.slu.se:1331 |
---|---|
record_format |
openpolar |
spelling |
ftslunivuppsala:oai:pub.epsilon.slu.se:1331 2024-06-09T07:44:43+00:00 Endocrine regulation of early sexual maturation in male Atlantic salmon parr Maugars, Gersende 2007-01 application/pdf https://pub.epsilon.slu.se/1331/ https://pub.epsilon.slu.se/1331/3/Summary_Maugars.pdf eng eng https://pub.epsilon.slu.se/1331/3/Summary_Maugars.pdf Maugars, Gersende (2007). Endocrine regulation of early sexual maturation in male Atlantic salmon parr. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 2007:7 ISBN 978-91-576-7306-0 [Doctoral thesis] Doctoral thesis NonPeerReviewed info:eu-repo/semantics/doctoralThesis 2007 ftslunivuppsala 2024-05-16T04:04:25Z This thesis deals with changes in gene expression during activation of the brain-pituitary-gonadal axis at puberty in early maturing male Atlantic salmon (Salmo salar) parr. To help elucidate the physiological roles of gonadotropins and their receptors in the regulation of puberty, cDNAs encoding FSH and LH receptors (FSHR and LHR, respectively) of Atlantic salmon were cloned and characterized. Gene expression of the receptors in the testes was analyzed in parallel with pituitary expression of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) β-subunit genes by RT-PCR. In addition, functional genes encoding proteins involved in the steroidogenic pathway and anti-Müllerian hormone (AMH) were studied in the testes, and plasma 11-ketostestosterone levels were measured. One-summer-old male Atlantic salmon parr were sampled from the prepubertal stage in December until spermiation in October. Sequence analysis of the Atlantic salmon FSHR and LHR showed the typical structure features of glycoprotein receptors including a large extracellular domain connected to a G protein-coupled transmembrane domain. Both of these gonadotropin receptors were expressed in immature testis, FSHR more abundantly than LHR. FSHR transcript levels increased in parallel with FSHβ levels from early spermatogenesis onwards while LHR mRNA levels started to increase prior to any major changes in LHβ expression. De novo transcription of genes encoding steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase, cytochrome P450 17α-hydroxylase/17,20-lyase, and 11β-hydroxysteroid dehydrogenase was observed during the initiation of spermatogenesis in parallel with the changes in FSHβ levels. In contrast, AMH expression was downregulated and AMH levels were lowest during spermiogenesis. During spermatogenesis, large increases in the expression of LHR and all of the steroidogenic genes studied occurred concomitantly with the rise in LHβ transcripts. These findings suggest that FSH is involved in regulation of the expression of ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
institution |
Open Polar |
collection |
Swedish University of Agricultural Sciences (SLU): Epsilon Open Archive |
op_collection_id |
ftslunivuppsala |
language |
English |
description |
This thesis deals with changes in gene expression during activation of the brain-pituitary-gonadal axis at puberty in early maturing male Atlantic salmon (Salmo salar) parr. To help elucidate the physiological roles of gonadotropins and their receptors in the regulation of puberty, cDNAs encoding FSH and LH receptors (FSHR and LHR, respectively) of Atlantic salmon were cloned and characterized. Gene expression of the receptors in the testes was analyzed in parallel with pituitary expression of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) β-subunit genes by RT-PCR. In addition, functional genes encoding proteins involved in the steroidogenic pathway and anti-Müllerian hormone (AMH) were studied in the testes, and plasma 11-ketostestosterone levels were measured. One-summer-old male Atlantic salmon parr were sampled from the prepubertal stage in December until spermiation in October. Sequence analysis of the Atlantic salmon FSHR and LHR showed the typical structure features of glycoprotein receptors including a large extracellular domain connected to a G protein-coupled transmembrane domain. Both of these gonadotropin receptors were expressed in immature testis, FSHR more abundantly than LHR. FSHR transcript levels increased in parallel with FSHβ levels from early spermatogenesis onwards while LHR mRNA levels started to increase prior to any major changes in LHβ expression. De novo transcription of genes encoding steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase, cytochrome P450 17α-hydroxylase/17,20-lyase, and 11β-hydroxysteroid dehydrogenase was observed during the initiation of spermatogenesis in parallel with the changes in FSHβ levels. In contrast, AMH expression was downregulated and AMH levels were lowest during spermiogenesis. During spermatogenesis, large increases in the expression of LHR and all of the steroidogenic genes studied occurred concomitantly with the rise in LHβ transcripts. These findings suggest that FSH is involved in regulation of the expression of ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Maugars, Gersende |
spellingShingle |
Maugars, Gersende Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
author_facet |
Maugars, Gersende |
author_sort |
Maugars, Gersende |
title |
Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
title_short |
Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
title_full |
Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
title_fullStr |
Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
title_full_unstemmed |
Endocrine regulation of early sexual maturation in male Atlantic salmon parr |
title_sort |
endocrine regulation of early sexual maturation in male atlantic salmon parr |
publishDate |
2007 |
url |
https://pub.epsilon.slu.se/1331/ https://pub.epsilon.slu.se/1331/3/Summary_Maugars.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://pub.epsilon.slu.se/1331/3/Summary_Maugars.pdf Maugars, Gersende (2007). Endocrine regulation of early sexual maturation in male Atlantic salmon parr. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 2007:7 ISBN 978-91-576-7306-0 [Doctoral thesis] |
_version_ |
1801373520120250368 |