Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi
Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyo...
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ftsissa:oai:iris.sissa.it:20.500.11767/85974 2023-05-15T15:48:00+02:00 Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thoma Selander, Erik Ferrante, Maria I 2018 https://hdl.handle.net/20.500.11767/85974 https://doi.org/10.1038/s41396-018-0094-0 eng eng info:eu-repo/semantics/altIdentifier/pmid/29599523 info:eu-repo/semantics/altIdentifier/wos/WOS:000432357500017 volume:12 issue:6 firstpage:1594 lastpage:1604 numberofpages:11 journal:THE ISME JOURNAL info:eu-repo/grantAgreement/EC/FP7/600407 http://hdl.handle.net/20.500.11767/85974 doi:10.1038/s41396-018-0094-0 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85044529293 info:eu-repo/semantics/openAccess Settore BIO/13 - Biologia Applicata info:eu-repo/semantics/article 2018 ftsissa https://doi.org/20.500.11767/8597410.1038/s41396-018-0094-0 2023-03-21T23:25:39Z Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. Article in Journal/Newspaper Calanus finmarchicus Copepods International School for Advanced Studies (SISSA), Trieste: SISSA Digital Library (SDL) The ISME Journal 12 6 1594 1604 |
institution |
Open Polar |
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International School for Advanced Studies (SISSA), Trieste: SISSA Digital Library (SDL) |
op_collection_id |
ftsissa |
language |
English |
topic |
Settore BIO/13 - Biologia Applicata |
spellingShingle |
Settore BIO/13 - Biologia Applicata Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
topic_facet |
Settore BIO/13 - Biologia Applicata |
description |
Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. |
author2 |
Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thoma Selander, Erik Ferrante, Maria I |
format |
Article in Journal/Newspaper |
author |
Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I |
author_facet |
Amato, Alberto Sabatino, Valeria Nylund, Göran M Bergkvist, Johanna Basu, Swaraj Andersson, Mats X Sanges, Remo Godhe, Anna Kiørboe, Thomas Selander, Erik Ferrante, Maria I |
author_sort |
Amato, Alberto |
title |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_short |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_fullStr |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full_unstemmed |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_sort |
grazer-induced transcriptomic and metabolomic response of the chain-forming diatom skeletonema marinoi |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.11767/85974 https://doi.org/10.1038/s41396-018-0094-0 |
genre |
Calanus finmarchicus Copepods |
genre_facet |
Calanus finmarchicus Copepods |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/29599523 info:eu-repo/semantics/altIdentifier/wos/WOS:000432357500017 volume:12 issue:6 firstpage:1594 lastpage:1604 numberofpages:11 journal:THE ISME JOURNAL info:eu-repo/grantAgreement/EC/FP7/600407 http://hdl.handle.net/20.500.11767/85974 doi:10.1038/s41396-018-0094-0 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85044529293 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11767/8597410.1038/s41396-018-0094-0 |
container_title |
The ISME Journal |
container_volume |
12 |
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6 |
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1594 |
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1604 |
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