Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua)
Due to the heavy fuel oil (HFO) ban in Arctic maritime transport and new legislations restricting the sulphur content of fuel oils, new fuel oil types are continuously developed. However, the potential impacts of these new fuel oil types on marine ecosystems during accidental spills are largely unkn...
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ftsintef:oai:sintef.brage.unit.no:11250/3025602 2023-05-15T14:59:53+02:00 Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) Hansen, Bjørn Henrik Nordtug, Trond Farkas, Julia Khan, Essa Ahsan Oteri, Erika Kvæstad, Bjarne Faksness, Liv-Guri Daling, Per Snorre Arukwe, Augustine 2021 application/pdf https://hdl.handle.net/11250/3025602 https://doi.org/10.1016/j.aquatox.2021.105881 eng eng Elsevier Aquatic Toxicology. 2021, 237 1-12. urn:issn:0166-445X https://hdl.handle.net/11250/3025602 https://doi.org/10.1016/j.aquatox.2021.105881 cristin:1921771 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Author(s). Published by Elsevier B.V. CC-BY 1-12 237 Aquatic Toxicology Cyp1a AhR gene battery Embryotoxicity Heavy fuel oil Gas oil Diesel Peer reviewed Journal article 2021 ftsintef https://doi.org/10.1016/j.aquatox.2021.105881 2022-10-19T22:42:51Z Due to the heavy fuel oil (HFO) ban in Arctic maritime transport and new legislations restricting the sulphur content of fuel oils, new fuel oil types are continuously developed. However, the potential impacts of these new fuel oil types on marine ecosystems during accidental spills are largely unknown. In this study, we studied the toxicity of three marine fuel oils (two marine gas oils with low sulphur contents and a heavy fuel oil) in early life stages of cod (Gadus morhua). Embryos were exposed for 4 days to water-soluble fractions of fuel oils at concentrations ranging from 4.1 - 128.3 µg TPAH/L, followed by recovery in clean seawater until 17 days post fertilization. Exposure to all three fuel oils resulted in developmental toxicity, including severe morphological changes, deformations and cardiotoxicity. To assess underlying molecular mechanisms, we studied fuel oil-mediated activation of aryl hydrocarbon receptor (Ahr) gene battery and genes related to cardiovascular, angiogenesis and osteogenesis pathways. Overall, our results suggest comparable mechanisms of toxicity for the three fuel oils. All fuel oils caused concentration-dependant increases of cyp1a mRNA which paralleled ahrr, but not ahr1b transcript expression. On the angiogenesis and osteogenesis pathways, fuel oils produced concentration-specific transcriptional effects that were either increasing or decreasing, compared to control embryos. Based on the observed toxic responses, toxicity threshold values were estimated for individual endpoints to assess the most sensitive molecular and physiological effects, suggesting that unresolved petrogenic components may be significant contributors to the observed toxicity. publishedVersion Article in Journal/Newspaper Arctic atlantic cod Gadus morhua SINTEF Open (Brage) Arctic Aquatic Toxicology 237 105881 |
institution |
Open Polar |
collection |
SINTEF Open (Brage) |
op_collection_id |
ftsintef |
language |
English |
topic |
Cyp1a AhR gene battery Embryotoxicity Heavy fuel oil Gas oil Diesel |
spellingShingle |
Cyp1a AhR gene battery Embryotoxicity Heavy fuel oil Gas oil Diesel Hansen, Bjørn Henrik Nordtug, Trond Farkas, Julia Khan, Essa Ahsan Oteri, Erika Kvæstad, Bjarne Faksness, Liv-Guri Daling, Per Snorre Arukwe, Augustine Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
topic_facet |
Cyp1a AhR gene battery Embryotoxicity Heavy fuel oil Gas oil Diesel |
description |
Due to the heavy fuel oil (HFO) ban in Arctic maritime transport and new legislations restricting the sulphur content of fuel oils, new fuel oil types are continuously developed. However, the potential impacts of these new fuel oil types on marine ecosystems during accidental spills are largely unknown. In this study, we studied the toxicity of three marine fuel oils (two marine gas oils with low sulphur contents and a heavy fuel oil) in early life stages of cod (Gadus morhua). Embryos were exposed for 4 days to water-soluble fractions of fuel oils at concentrations ranging from 4.1 - 128.3 µg TPAH/L, followed by recovery in clean seawater until 17 days post fertilization. Exposure to all three fuel oils resulted in developmental toxicity, including severe morphological changes, deformations and cardiotoxicity. To assess underlying molecular mechanisms, we studied fuel oil-mediated activation of aryl hydrocarbon receptor (Ahr) gene battery and genes related to cardiovascular, angiogenesis and osteogenesis pathways. Overall, our results suggest comparable mechanisms of toxicity for the three fuel oils. All fuel oils caused concentration-dependant increases of cyp1a mRNA which paralleled ahrr, but not ahr1b transcript expression. On the angiogenesis and osteogenesis pathways, fuel oils produced concentration-specific transcriptional effects that were either increasing or decreasing, compared to control embryos. Based on the observed toxic responses, toxicity threshold values were estimated for individual endpoints to assess the most sensitive molecular and physiological effects, suggesting that unresolved petrogenic components may be significant contributors to the observed toxicity. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Hansen, Bjørn Henrik Nordtug, Trond Farkas, Julia Khan, Essa Ahsan Oteri, Erika Kvæstad, Bjarne Faksness, Liv-Guri Daling, Per Snorre Arukwe, Augustine |
author_facet |
Hansen, Bjørn Henrik Nordtug, Trond Farkas, Julia Khan, Essa Ahsan Oteri, Erika Kvæstad, Bjarne Faksness, Liv-Guri Daling, Per Snorre Arukwe, Augustine |
author_sort |
Hansen, Bjørn Henrik |
title |
Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
title_short |
Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
title_full |
Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
title_fullStr |
Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Toxicity and developmental effects of Arctic fuel oil types on early life stages of Atlantic cod (Gadus morhua) |
title_sort |
toxicity and developmental effects of arctic fuel oil types on early life stages of atlantic cod (gadus morhua) |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/3025602 https://doi.org/10.1016/j.aquatox.2021.105881 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic atlantic cod Gadus morhua |
genre_facet |
Arctic atlantic cod Gadus morhua |
op_source |
1-12 237 Aquatic Toxicology |
op_relation |
Aquatic Toxicology. 2021, 237 1-12. urn:issn:0166-445X https://hdl.handle.net/11250/3025602 https://doi.org/10.1016/j.aquatox.2021.105881 cristin:1921771 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no © 2021 The Author(s). Published by Elsevier B.V. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.aquatox.2021.105881 |
container_title |
Aquatic Toxicology |
container_volume |
237 |
container_start_page |
105881 |
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1766331996093546496 |