Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice
The anthropogenic release of oil hydrocarbons into the cold marine environment is an increasing concern due to the elevated usage of sea routes and the exploration of new oil drilling sites in Arctic areas. The aim of this study was to evaluate prokaryotic community structures and the genetic potent...
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Online Access: | https://hdl.handle.net/11250/2995635 https://doi.org/10.3390/ microorganisms10020328 |
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ftsintef:oai:sintef.brage.unit.no:11250/2995635 2023-05-15T14:56:42+02:00 Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice Peeb, Angela Dang, Nga Truu, Marika Hiie, Nõlvak Petrich, Chris Truu, Jaak 2022 application/pdf https://hdl.handle.net/11250/2995635 https://doi.org/10.3390/ microorganisms10020328 eng eng EC/H2020/Project GRACE Grant N. 679266 Andre: The Estonian Research Council, grant PRG548 Microorganisms. 2022, 10 (328), . urn:issn:2076-2607 https://hdl.handle.net/11250/2995635 https://doi.org/10.3390/ microorganisms10020328 cristin:2023608 24 10 Microorganisms 328 Prokaryotic community Arctic seawater Sea ice Crude oil VDP::Bioteknologi: 590 VDP::Biotechnology: 590 Peer reviewed Journal article 2022 ftsintef 2022-05-25T22:41:00Z The anthropogenic release of oil hydrocarbons into the cold marine environment is an increasing concern due to the elevated usage of sea routes and the exploration of new oil drilling sites in Arctic areas. The aim of this study was to evaluate prokaryotic community structures and the genetic potential of hydrocarbon degradation in the metagenomes of seawater, sea ice, and crude oil encapsulating the sea ice of the Norwegian fjord, Ofotfjorden. Although the results indicated substantial differences between the structure of prokaryotic communities in seawater and sea ice, the crude oil encapsulating sea ice (SIO) showed increased abundances of many genera-containing hydrocarbon-degrading organisms, including Bermanella, Colwellia, and Glaciecola. Although the metagenome of seawater was rich in a variety of hydrocarbon degradation-related functional genes (HDGs) associated with the metabolism of n-alkanes, and mono- and polyaromatic hydrocarbons, most of the normalized gene counts were highest in the clean sea ice metagenome, whereas in SIO, these counts were the lowest. The long-chain alkane degradation gene almA was detected from all the studied metagenomes and its counts exceeded ladA and alkB counts in both sea ice metagenomes. In addition, almA was related to the most diverse group of prokaryotic genera. Almost all 18 goodand high-quality metagenome-assembled genomes (MAGs) had diverse HDGs profiles. The MAGs recovered from the SIO metagenome belonged to the abundant taxa, such as Glaciecola, Bermanella, and Rhodobacteracea, in this environment. The genera associated with HDGs were often previously known as hydrocarbon-degrading genera. However, a substantial number of new associations, either between already known hydrocarbon-degrading genera and new HDGs or between genera not known to contain hydrocarbon degraders and multiple HDGs, were found. The superimposition of the results of comparing HDG associations with taxonomy, the HDG profiles of MAGs, and the full genomes of organisms in the KEGG database ... Article in Journal/Newspaper Arctic Ofotfjorden Sea ice SINTEF Open (Brage) Arctic Ofotfjorden ENVELOPE(17.015,17.015,68.420,68.420) |
institution |
Open Polar |
collection |
SINTEF Open (Brage) |
op_collection_id |
ftsintef |
language |
English |
topic |
Prokaryotic community Arctic seawater Sea ice Crude oil VDP::Bioteknologi: 590 VDP::Biotechnology: 590 |
spellingShingle |
Prokaryotic community Arctic seawater Sea ice Crude oil VDP::Bioteknologi: 590 VDP::Biotechnology: 590 Peeb, Angela Dang, Nga Truu, Marika Hiie, Nõlvak Petrich, Chris Truu, Jaak Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
topic_facet |
Prokaryotic community Arctic seawater Sea ice Crude oil VDP::Bioteknologi: 590 VDP::Biotechnology: 590 |
description |
The anthropogenic release of oil hydrocarbons into the cold marine environment is an increasing concern due to the elevated usage of sea routes and the exploration of new oil drilling sites in Arctic areas. The aim of this study was to evaluate prokaryotic community structures and the genetic potential of hydrocarbon degradation in the metagenomes of seawater, sea ice, and crude oil encapsulating the sea ice of the Norwegian fjord, Ofotfjorden. Although the results indicated substantial differences between the structure of prokaryotic communities in seawater and sea ice, the crude oil encapsulating sea ice (SIO) showed increased abundances of many genera-containing hydrocarbon-degrading organisms, including Bermanella, Colwellia, and Glaciecola. Although the metagenome of seawater was rich in a variety of hydrocarbon degradation-related functional genes (HDGs) associated with the metabolism of n-alkanes, and mono- and polyaromatic hydrocarbons, most of the normalized gene counts were highest in the clean sea ice metagenome, whereas in SIO, these counts were the lowest. The long-chain alkane degradation gene almA was detected from all the studied metagenomes and its counts exceeded ladA and alkB counts in both sea ice metagenomes. In addition, almA was related to the most diverse group of prokaryotic genera. Almost all 18 goodand high-quality metagenome-assembled genomes (MAGs) had diverse HDGs profiles. The MAGs recovered from the SIO metagenome belonged to the abundant taxa, such as Glaciecola, Bermanella, and Rhodobacteracea, in this environment. The genera associated with HDGs were often previously known as hydrocarbon-degrading genera. However, a substantial number of new associations, either between already known hydrocarbon-degrading genera and new HDGs or between genera not known to contain hydrocarbon degraders and multiple HDGs, were found. The superimposition of the results of comparing HDG associations with taxonomy, the HDG profiles of MAGs, and the full genomes of organisms in the KEGG database ... |
format |
Article in Journal/Newspaper |
author |
Peeb, Angela Dang, Nga Truu, Marika Hiie, Nõlvak Petrich, Chris Truu, Jaak |
author_facet |
Peeb, Angela Dang, Nga Truu, Marika Hiie, Nõlvak Petrich, Chris Truu, Jaak |
author_sort |
Peeb, Angela |
title |
Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
title_short |
Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
title_full |
Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
title_fullStr |
Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
title_full_unstemmed |
Assessment of Hydrocarbon Degradation Potential in Microbial Communities in Arctic Sea Ice |
title_sort |
assessment of hydrocarbon degradation potential in microbial communities in arctic sea ice |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/2995635 https://doi.org/10.3390/ microorganisms10020328 |
long_lat |
ENVELOPE(17.015,17.015,68.420,68.420) |
geographic |
Arctic Ofotfjorden |
geographic_facet |
Arctic Ofotfjorden |
genre |
Arctic Ofotfjorden Sea ice |
genre_facet |
Arctic Ofotfjorden Sea ice |
op_source |
24 10 Microorganisms 328 |
op_relation |
EC/H2020/Project GRACE Grant N. 679266 Andre: The Estonian Research Council, grant PRG548 Microorganisms. 2022, 10 (328), . urn:issn:2076-2607 https://hdl.handle.net/11250/2995635 https://doi.org/10.3390/ microorganisms10020328 cristin:2023608 |
_version_ |
1766328785356980224 |