Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity
Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual isolates originating from biota, water and sediment samples taken at a various d...
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ftsintef:oai:sintef.brage.unit.no:11250/2576209 2024-09-15T17:58:02+00:00 Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity Shojaei Moghadam, Morteza Albersmeier, Andreas Winkler, Anika Cimmino, Lorenzo Rise, Kjersti Hohmann-Marriott, Martin Frank Kalinowski, Jörn Rückert, Christian Wentzel, Alexander Lale, Rahmi 2016 application/pdf http://hdl.handle.net/11250/2576209 https://doi.org/10.1186/s12864-016-2445-4 eng eng BMC Genomics. 2016, 17 (117), . urn:issn:1471-2164 http://hdl.handle.net/11250/2576209 https://doi.org/10.1186/s12864-016-2445-4 cristin:1342156 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no 14 17 BMC Genomics 117 Journal article Peer reviewed 2016 ftsintef https://doi.org/10.1186/s12864-016-2445-4 2024-08-30T03:27:11Z Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual isolates originating from biota, water and sediment samples taken at a various depth in the Barents Sea, North of mainland Norway, with an all year round seawater temperature of 4 °C. The entire collection was subjected to high-throughput screening for detection of extracellular laccase activity with guaiacol as a substrate. Results In total, 13 laccase-positive isolates were identified, all belonging to the Psychrobacter genus. From the most diverse four strains, based on 16S rRNA gene sequence analysis, all originating from the same Botryllus sp. colonial ascidian tunicate sample, genomic DNA was isolated and genome sequenced using a combined approach of whole genome shotgun and 8 kb mate-pair library sequencing on an Illumina MiSeq platform. The genomes were assembled and revealed genome sizes between 3.29 and 3.52 Mbp with an average G + C content of around 42 %, with one to seven plasmids present in the four strains. Bioinformatics based genome mining was performed to describe the metabolic potential of these four strains and to identify gene candidates potentially responsible for the observed laccase-positive phenotype. Up to two different laccase-like multicopper oxidase (LMCO) encoding gene candidates were identified in each of the four strains. Heterologous expression of P11F6-LMCO and P11G5-LMCO2 in Escherichia coli BL21 (DE3) resulted in recombinant proteins exhibiting 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and guaiacol oxidizing activity. Conclusions Thirteen Psychrobacter species with laccase-positive phenotype were isolated from a collection of Arctic marine bacteria. Four of the isolates were genome sequenced. The overall genome features were similar to other publicly available Psychrobacter genome sequences except for P11G5 harboring seven plasmids. However, ... Article in Journal/Newspaper Barents Sea SINTEF Open BMC Genomics 17 1 |
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English |
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Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual isolates originating from biota, water and sediment samples taken at a various depth in the Barents Sea, North of mainland Norway, with an all year round seawater temperature of 4 °C. The entire collection was subjected to high-throughput screening for detection of extracellular laccase activity with guaiacol as a substrate. Results In total, 13 laccase-positive isolates were identified, all belonging to the Psychrobacter genus. From the most diverse four strains, based on 16S rRNA gene sequence analysis, all originating from the same Botryllus sp. colonial ascidian tunicate sample, genomic DNA was isolated and genome sequenced using a combined approach of whole genome shotgun and 8 kb mate-pair library sequencing on an Illumina MiSeq platform. The genomes were assembled and revealed genome sizes between 3.29 and 3.52 Mbp with an average G + C content of around 42 %, with one to seven plasmids present in the four strains. Bioinformatics based genome mining was performed to describe the metabolic potential of these four strains and to identify gene candidates potentially responsible for the observed laccase-positive phenotype. Up to two different laccase-like multicopper oxidase (LMCO) encoding gene candidates were identified in each of the four strains. Heterologous expression of P11F6-LMCO and P11G5-LMCO2 in Escherichia coli BL21 (DE3) resulted in recombinant proteins exhibiting 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and guaiacol oxidizing activity. Conclusions Thirteen Psychrobacter species with laccase-positive phenotype were isolated from a collection of Arctic marine bacteria. Four of the isolates were genome sequenced. The overall genome features were similar to other publicly available Psychrobacter genome sequences except for P11G5 harboring seven plasmids. However, ... |
format |
Article in Journal/Newspaper |
author |
Shojaei Moghadam, Morteza Albersmeier, Andreas Winkler, Anika Cimmino, Lorenzo Rise, Kjersti Hohmann-Marriott, Martin Frank Kalinowski, Jörn Rückert, Christian Wentzel, Alexander Lale, Rahmi |
spellingShingle |
Shojaei Moghadam, Morteza Albersmeier, Andreas Winkler, Anika Cimmino, Lorenzo Rise, Kjersti Hohmann-Marriott, Martin Frank Kalinowski, Jörn Rückert, Christian Wentzel, Alexander Lale, Rahmi Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
author_facet |
Shojaei Moghadam, Morteza Albersmeier, Andreas Winkler, Anika Cimmino, Lorenzo Rise, Kjersti Hohmann-Marriott, Martin Frank Kalinowski, Jörn Rückert, Christian Wentzel, Alexander Lale, Rahmi |
author_sort |
Shojaei Moghadam, Morteza |
title |
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
title_short |
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
title_full |
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
title_fullStr |
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
title_full_unstemmed |
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity |
title_sort |
isolation and genome sequencing of four arctic marine psychrobacter strains exhibiting multicopper oxidase activity |
publishDate |
2016 |
url |
http://hdl.handle.net/11250/2576209 https://doi.org/10.1186/s12864-016-2445-4 |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
14 17 BMC Genomics 117 |
op_relation |
BMC Genomics. 2016, 17 (117), . urn:issn:1471-2164 http://hdl.handle.net/11250/2576209 https://doi.org/10.1186/s12864-016-2445-4 cristin:1342156 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_doi |
https://doi.org/10.1186/s12864-016-2445-4 |
container_title |
BMC Genomics |
container_volume |
17 |
container_issue |
1 |
_version_ |
1810434232371118080 |