Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels
Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synt...
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Online Access: | http://hdl.handle.net/11250/2459957 https://doi.org/10.1016/j.envpol.2016.09.067 |
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ftsintef:oai:sintef.brage.unit.no:11250/2459957 2023-05-15T18:15:46+02:00 Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Stanková, Radka Ciesielski, Tomasz Maciej Olsen, Anders Johny Posch, Wilfried Flaten, Trond Peder Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn Munro 2017 application/pdf http://hdl.handle.net/11250/2459957 https://doi.org/10.1016/j.envpol.2016.09.067 eng eng Elsevier Environmental Pollution. 2017, 220 328-336. urn:issn:0269-7491 http://hdl.handle.net/11250/2459957 https://doi.org/10.1016/j.envpol.2016.09.067 cristin:1420561 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no © 2016 Elsevier Ltd. All rights reserved. © 2016 postprint the authors CC-BY-NC-ND 328-336 220 Environmental Pollution Androgens Estradiol Silver nanoparticles Mixed exposure Vitellogenin Testosterone Journal article Peer reviewed 2017 ftsintef https://doi.org/10.1016/j.envpol.2016.09.067 2021-08-04T11:59:19Z Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17a-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 mg L 1 and 200 mg L 1), Agþ (50 mg L 1), EE2 (50 ng L 1) and AgNP þ EE2 (2 or 200 mg L 1þ50 ng L 1). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP þ EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Agþ exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. acceptedVersion Article in Journal/Newspaper Scophthalmus maximus Turbot SINTEF Open (Brage) Environmental Pollution 220 328 336 |
institution |
Open Polar |
collection |
SINTEF Open (Brage) |
op_collection_id |
ftsintef |
language |
English |
topic |
Androgens Estradiol Silver nanoparticles Mixed exposure Vitellogenin Testosterone |
spellingShingle |
Androgens Estradiol Silver nanoparticles Mixed exposure Vitellogenin Testosterone Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Stanková, Radka Ciesielski, Tomasz Maciej Olsen, Anders Johny Posch, Wilfried Flaten, Trond Peder Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn Munro Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
topic_facet |
Androgens Estradiol Silver nanoparticles Mixed exposure Vitellogenin Testosterone |
description |
Combined exposure to engineered nanoparticles (ENPs) and anthropogenic contaminants can lead to changes in bioavailability, uptake and thus effects of both groups of contaminants. In this study we investigated effects of single and combined exposures of silver (Ag) nanoparticles (AgNPs) and the synthetic hormone 17a-ethinylestradiol (EE2) on tissue uptake of both contaminants in juvenile turbot (Scophthalmus maximus). Silver uptake and tissue distribution (gills, liver, kidney, stomach, muscle and bile) were analyzed following a 14-day, 2-h daily pulsed exposure to AgNPs (2 mg L 1 and 200 mg L 1), Agþ (50 mg L 1), EE2 (50 ng L 1) and AgNP þ EE2 (2 or 200 mg L 1þ50 ng L 1). Effects of the exposures on plasma vitellogenin (Vtg) levels, EE2 and steroid hormone concentrations were investigated. The AgNP and AgNP þ EE2 exposures resulted in similar Ag concentrations in the tissues, indicating that combined exposure did not influence Ag uptake in tissues. The highest Ag concentrations were found in gills. For the Agþ exposed fish, the highest Ag concentrations were measured in the liver. Our results show dissolution processes of AgNPs in seawater, indicating that the tissue concentrations of Ag may partly originate from ionic release. Plasma EE2 concentrations and Vtg induction were similar in fish exposed to the single contaminants and the mixed contaminants, indicating that the presence of AgNPs did not significantly alter EE2 uptake. Similarly, concentrations of most steroid hormones were not significantly altered due to exposures to the combined contaminants versus the single compound exposures. However, high concentrations of AgNPs in combination with EE2 caused a drop of estrone (E1) (female fish) and androstenedione (AN) (male and female fish) levels in plasma below quantification limits. Our results indicate that the interactive effects between AgNPs and EE2 are limited, with only high concentrations of AgNPs triggering synergistic effects on plasma steroid hormone concentrations in juvenile turbots. acceptedVersion |
format |
Article in Journal/Newspaper |
author |
Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Stanková, Radka Ciesielski, Tomasz Maciej Olsen, Anders Johny Posch, Wilfried Flaten, Trond Peder Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn Munro |
author_facet |
Farkas, Julia Salaberria, Iurgi Styrishave, Bjarne Stanková, Radka Ciesielski, Tomasz Maciej Olsen, Anders Johny Posch, Wilfried Flaten, Trond Peder Krøkje, Åse Salvenmoser, Willi Jenssen, Bjørn Munro |
author_sort |
Farkas, Julia |
title |
Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
title_short |
Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
title_full |
Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
title_fullStr |
Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
title_full_unstemmed |
Exposure of juvenile turbot (Scophthalmus maximus) to AgNP-EE2 mixtures: Implications on contaminant bioavailability and plasma steroid hormone levels |
title_sort |
exposure of juvenile turbot (scophthalmus maximus) to agnp-ee2 mixtures: implications on contaminant bioavailability and plasma steroid hormone levels |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/11250/2459957 https://doi.org/10.1016/j.envpol.2016.09.067 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
328-336 220 Environmental Pollution |
op_relation |
Environmental Pollution. 2017, 220 328-336. urn:issn:0269-7491 http://hdl.handle.net/11250/2459957 https://doi.org/10.1016/j.envpol.2016.09.067 cristin:1420561 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no © 2016 Elsevier Ltd. All rights reserved. © 2016 postprint the authors |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.envpol.2016.09.067 |
container_title |
Environmental Pollution |
container_volume |
220 |
container_start_page |
328 |
op_container_end_page |
336 |
_version_ |
1766188975146401792 |