Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon
Atlantic salmon depend on genetic cues to determine whether an individual is male or female. A novel sex-determining gene, sexually dimorphic on the Y chromosome (sdY), is found exclusively in all salmonids. Unlike other sex-determining genes, sdY lacks a DNA-binding domain. Instead, it is a diverge...
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ftsimonfu:oai:summit.sfu.ca:17172 2023-05-15T15:31:22+02:00 Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon Stadnik, Agnieszka 2017-01-20 http://summit.sfu.ca/item/17172 unknown etd10002 http://summit.sfu.ca/item/17172 Thesis 2017 ftsimonfu 2022-04-07T18:40:58Z Atlantic salmon depend on genetic cues to determine whether an individual is male or female. A novel sex-determining gene, sexually dimorphic on the Y chromosome (sdY), is found exclusively in all salmonids. Unlike other sex-determining genes, sdY lacks a DNA-binding domain. Instead, it is a divergent, truncated form of interferon regulatory factor 9. As a recently discovered gene, little is known about sdY; how it is involved in sex-determination and what proteins interact with it. Identification of protein interactors was done through a variety of techniques including yeast two-hybridization, co-immunoprecipitation and histidine-tagged pull down assays. These assays identified several proteins: SdY itself, 40S ribosomal protein S16 and SA, isocitrate dehydrogenase, heat shock protein HSP 90-beta, and ras GTPase-activating-like protein IQGAP1, as well as creatine kinase, GDP-mannose-4,6-dehydratase, sodium/potassium-transporting ATPase subunit alpha-1, AP-1 complex subunit beta-1, and hydroxysteroid dehydrogenase (17-beta) 4. The yeast two-hybrid assay also identified 3’ UTR of annexin A7-like and transmembrane protein 91-like, most likely false positives. This broad range of candidates has led me to believe that SdY is involved either in the biosynthesis of testosterone or in the testosterone signalling pathway. Thesis Atlantic salmon Summit - SFU Research Repository (Simon Fraser University) The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) |
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Open Polar |
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Summit - SFU Research Repository (Simon Fraser University) |
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ftsimonfu |
language |
unknown |
description |
Atlantic salmon depend on genetic cues to determine whether an individual is male or female. A novel sex-determining gene, sexually dimorphic on the Y chromosome (sdY), is found exclusively in all salmonids. Unlike other sex-determining genes, sdY lacks a DNA-binding domain. Instead, it is a divergent, truncated form of interferon regulatory factor 9. As a recently discovered gene, little is known about sdY; how it is involved in sex-determination and what proteins interact with it. Identification of protein interactors was done through a variety of techniques including yeast two-hybridization, co-immunoprecipitation and histidine-tagged pull down assays. These assays identified several proteins: SdY itself, 40S ribosomal protein S16 and SA, isocitrate dehydrogenase, heat shock protein HSP 90-beta, and ras GTPase-activating-like protein IQGAP1, as well as creatine kinase, GDP-mannose-4,6-dehydratase, sodium/potassium-transporting ATPase subunit alpha-1, AP-1 complex subunit beta-1, and hydroxysteroid dehydrogenase (17-beta) 4. The yeast two-hybrid assay also identified 3’ UTR of annexin A7-like and transmembrane protein 91-like, most likely false positives. This broad range of candidates has led me to believe that SdY is involved either in the biosynthesis of testosterone or in the testosterone signalling pathway. |
format |
Thesis |
author |
Stadnik, Agnieszka |
spellingShingle |
Stadnik, Agnieszka Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
author_facet |
Stadnik, Agnieszka |
author_sort |
Stadnik, Agnieszka |
title |
Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
title_short |
Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
title_full |
Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
title_fullStr |
Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
title_full_unstemmed |
Candidate protein interactors of sexually dimorphic on the Y chromosome (SdY) in Atlantic salmon |
title_sort |
candidate protein interactors of sexually dimorphic on the y chromosome (sdy) in atlantic salmon |
publishDate |
2017 |
url |
http://summit.sfu.ca/item/17172 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
The ''Y'' |
geographic_facet |
The ''Y'' |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
etd10002 http://summit.sfu.ca/item/17172 |
_version_ |
1766361866356916224 |