Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica

Lake Vanda is a meromictic, permanently ice-covered lake in the McMurdo Dry Valleys. The McMurdo Dry Valleys are a polar desert in Southern Victorialand Antarctica. This area experiences very little rainfall and very cold average temperatures, around –20°C. Lake Vanda has an unusual limnological pro...

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Main Author: Tregoning, George Seibert
Format: Text
Language:unknown
Published: OpenSIUC 2010
Subjects:
Online Access:https://opensiuc.lib.siu.edu/theses/395
https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1402&context=theses
id ftsilluniv:oai:opensiuc.lib.siu.edu:theses-1402
record_format openpolar
spelling ftsilluniv:oai:opensiuc.lib.siu.edu:theses-1402 2023-05-15T13:56:48+02:00 Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica Tregoning, George Seibert 2010-12-01T08:00:00Z application/pdf https://opensiuc.lib.siu.edu/theses/395 https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1402&context=theses unknown OpenSIUC https://opensiuc.lib.siu.edu/theses/395 https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1402&context=theses Theses Antarctica Chromohalobacter Halophiles Lake Vanda McMurdo text 2010 ftsilluniv 2021-09-30T20:29:29Z Lake Vanda is a meromictic, permanently ice-covered lake in the McMurdo Dry Valleys. The McMurdo Dry Valleys are a polar desert in Southern Victorialand Antarctica. This area experiences very little rainfall and very cold average temperatures, around –20°C. Lake Vanda has an unusual limnological profile, with a sharp physical and chemical gradient at about 60 m where the water transitions from cold, oxic, and fresh, to warm, hypersaline, and sulfidic; CaCl2 rather than NaCl is the dominant salt. Aerobic enrichment techniques were used to isolate what turned out to be several strains of a species of Chromohalobacter, a genus of the Gammaproteobacteria, from Lake Vanda deep waters, while targeted enrichments for anaerobic and spore-forming bacteria were negative. The isolates were characterized for their temperature and pH optima, carbon and nitrogen nutrition, and salt tolerance and requirements. The results showed the organisms to be obligately aerobic with a broad pH range (optima pH 7). The isolates used some sugars and organic acids but not alcohols or fatty acids for energy and cell carbon, and showed a moderate requirement for NaCl but no requirement for CaCl2, even though CaCl2 is the predominant salt in their environment. Text Antarc* Antarctica McMurdo Dry Valleys polar desert Southern Illinois University Carbondale: OpenSUIC McMurdo Dry Valleys Vanda ENVELOPE(161.550,161.550,-77.533,-77.533) Lake Vanda ENVELOPE(161.600,161.600,-77.517,-77.517)
institution Open Polar
collection Southern Illinois University Carbondale: OpenSUIC
op_collection_id ftsilluniv
language unknown
topic Antarctica
Chromohalobacter
Halophiles
Lake Vanda
McMurdo
spellingShingle Antarctica
Chromohalobacter
Halophiles
Lake Vanda
McMurdo
Tregoning, George Seibert
Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
topic_facet Antarctica
Chromohalobacter
Halophiles
Lake Vanda
McMurdo
description Lake Vanda is a meromictic, permanently ice-covered lake in the McMurdo Dry Valleys. The McMurdo Dry Valleys are a polar desert in Southern Victorialand Antarctica. This area experiences very little rainfall and very cold average temperatures, around –20°C. Lake Vanda has an unusual limnological profile, with a sharp physical and chemical gradient at about 60 m where the water transitions from cold, oxic, and fresh, to warm, hypersaline, and sulfidic; CaCl2 rather than NaCl is the dominant salt. Aerobic enrichment techniques were used to isolate what turned out to be several strains of a species of Chromohalobacter, a genus of the Gammaproteobacteria, from Lake Vanda deep waters, while targeted enrichments for anaerobic and spore-forming bacteria were negative. The isolates were characterized for their temperature and pH optima, carbon and nitrogen nutrition, and salt tolerance and requirements. The results showed the organisms to be obligately aerobic with a broad pH range (optima pH 7). The isolates used some sugars and organic acids but not alcohols or fatty acids for energy and cell carbon, and showed a moderate requirement for NaCl but no requirement for CaCl2, even though CaCl2 is the predominant salt in their environment.
format Text
author Tregoning, George Seibert
author_facet Tregoning, George Seibert
author_sort Tregoning, George Seibert
title Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
title_short Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
title_full Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
title_fullStr Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
title_full_unstemmed Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica
title_sort isolation and characterization of halophilic heterotrophic bacteria from lake vanda, mcmurdo dry valleys, antarctica
publisher OpenSIUC
publishDate 2010
url https://opensiuc.lib.siu.edu/theses/395
https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1402&context=theses
long_lat ENVELOPE(161.550,161.550,-77.533,-77.533)
ENVELOPE(161.600,161.600,-77.517,-77.517)
geographic McMurdo Dry Valleys
Vanda
Lake Vanda
geographic_facet McMurdo Dry Valleys
Vanda
Lake Vanda
genre Antarc*
Antarctica
McMurdo Dry Valleys
polar desert
genre_facet Antarc*
Antarctica
McMurdo Dry Valleys
polar desert
op_source Theses
op_relation https://opensiuc.lib.siu.edu/theses/395
https://opensiuc.lib.siu.edu/cgi/viewcontent.cgi?article=1402&context=theses
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