Stepwise large genome assembly approach: a case of Siberian larch (Larix sibirica Ledeb)

• Published in: BMC Bioinformatics
• Main Authors: Dmitry A. Kuzmin, Sergey I. Feranchuk, Vadim V. Sharov, Alexander N. Cybin, Stepan V. Makolov, Yuliya A. Putintseva, Natalya V. Oreshkova, Konstantin V. Krutovsky
• Other Authors: Институт космических и информационных технологий, Институт фундаментальной биологии и биотехнологии, Кафедра высокопроизводительных вычислений, Базовая кафедра защиты и современных технологии мониторинга лесов
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: 2019
• Subjects: 34.03.23; Handle The; Sibirica
• Online Access: https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2570-y; http://elib.sfu-kras.ru/handle/2311/129305; https://doi.org/10.1186/s12859-018-2570-y

Background De novo assembling of large genomes, such as in conifers (~ 12–30 Gbp), which also consist of ~ 80% of repetitive DNA, is a very complex and computationally intense endeavor. One of the main problems in assembling such genomes lays in computing limitations of nucleotide sequence assembly...