Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen
Two Gram-stain-negative, facultative anaerobic chemoheterotrophic, pink-coloured, rod-shaped and non-motile bacterial strains, PAMC 29128 and PAMC 29148(T), were isolated from lichen. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strains PAMC 29128 and PAMC 29148(T) belong...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Online Access: | https://hdl.handle.net/10371/198094 https://doi.org/10.1099/ijsem.0.003695 |
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ftseoulnuniv:oai:s-space.snu.ac.kr:10371/198094 2024-01-14T09:59:59+01:00 Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen Noh, Hyun-Ju Baek, Kiwoon Hwang, Chung Yeon Shin, Seung Chul Hong, Soon Gyu Lee, Yung Mi Hwang, Chung Yeon 2020-11-18 https://hdl.handle.net/10371/198094 https://doi.org/10.1099/ijsem.0.003695 영어 unknown Society for General Microbiology International Journal of Systematic and Evolutionary Microbiology, Vol.69 No.12, pp.3837-3842 1466-5026 https://hdl.handle.net/10371/198094 doi:10.1099/ijsem.0.003695 000500924000027 2-s2.0-85076125291 116614 BACTERIAL COMMUNITIES GEOGRAPHY DATABASE TOOL DNA Lichenihabitans psoromatis polyphasic taxonomy lichen Antarctica new species Article ART 2020 ftseoulnuniv https://doi.org/10.1099/ijsem.0.003695 2023-12-15T01:40:14Z Two Gram-stain-negative, facultative anaerobic chemoheterotrophic, pink-coloured, rod-shaped and non-motile bacterial strains, PAMC 29128 and PAMC 29148(T), were isolated from lichen. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strains PAMC 29128 and PAMC 29148(T) belong to lichen-associated Rhizobiales-1 (LAR1), an uncultured phylogenetic lineage of the order Rhizobiales and the most closely related genera were Methylocapsa (<93.9 %) and Methylosinus (<93.8 %). The results of phylogenomic and genomic relatedness analyses also showed that strains PAMC 29128 and PAMC 29148(T) were clearly distinguished from other species in the order Rhizobiales with average nucleotide identity values of <71.4% and genome-to-genome distance values of <22.7 %. Genomic analysis revealed that strains PAMC 29128 and PAMC 29148(T) did not contain genes involved in atmospheric nitrogen fixation or utilization of carbon compounds such as methane and methanol. Strains PAMC 29128 and PAMC 29148(T) were able to utilize certain monosaccharides, disaccharides, sugar alcohols and other organic compounds as a sole carbon source. The major fatty acids (>10%) were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c; 33.7-39.7 %), summed feature 3 (C-16:1 omega 7c and/or C-16(:1) omega 6c; 25.2-254%) and C-19:0 cyclo omega 8c (11.9-15.4%). The major respiratory quinone was Q-10. The genomic DNA G+C contents of PAMC 29128 and PAMC 29148(T) were 63.0 and 63.1 mol%, respectively. Their distinct phylogenetic position and some physiological characteristics support the proposal of Lichenihabitans gen. nov., with the type species Lichenihabitans psoromatis sp. nov. (type strain, PAMC 29148(T)=KCCM 43293(T)=JCM 33311(T)). Lichenihabitantaceae fam. nov. is also proposed. Y 1 Article in Journal/Newspaper Antarc* Antarctic Antarctica Seoul National University: S-Space Antarctic International Journal of Systematic and Evolutionary Microbiology 69 12 3837 3842 |
institution |
Open Polar |
collection |
Seoul National University: S-Space |
op_collection_id |
ftseoulnuniv |
language |
unknown |
topic |
BACTERIAL COMMUNITIES GEOGRAPHY DATABASE TOOL DNA Lichenihabitans psoromatis polyphasic taxonomy lichen Antarctica new species |
spellingShingle |
BACTERIAL COMMUNITIES GEOGRAPHY DATABASE TOOL DNA Lichenihabitans psoromatis polyphasic taxonomy lichen Antarctica new species Noh, Hyun-Ju Baek, Kiwoon Hwang, Chung Yeon Shin, Seung Chul Hong, Soon Gyu Lee, Yung Mi Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
topic_facet |
BACTERIAL COMMUNITIES GEOGRAPHY DATABASE TOOL DNA Lichenihabitans psoromatis polyphasic taxonomy lichen Antarctica new species |
description |
Two Gram-stain-negative, facultative anaerobic chemoheterotrophic, pink-coloured, rod-shaped and non-motile bacterial strains, PAMC 29128 and PAMC 29148(T), were isolated from lichen. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strains PAMC 29128 and PAMC 29148(T) belong to lichen-associated Rhizobiales-1 (LAR1), an uncultured phylogenetic lineage of the order Rhizobiales and the most closely related genera were Methylocapsa (<93.9 %) and Methylosinus (<93.8 %). The results of phylogenomic and genomic relatedness analyses also showed that strains PAMC 29128 and PAMC 29148(T) were clearly distinguished from other species in the order Rhizobiales with average nucleotide identity values of <71.4% and genome-to-genome distance values of <22.7 %. Genomic analysis revealed that strains PAMC 29128 and PAMC 29148(T) did not contain genes involved in atmospheric nitrogen fixation or utilization of carbon compounds such as methane and methanol. Strains PAMC 29128 and PAMC 29148(T) were able to utilize certain monosaccharides, disaccharides, sugar alcohols and other organic compounds as a sole carbon source. The major fatty acids (>10%) were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c; 33.7-39.7 %), summed feature 3 (C-16:1 omega 7c and/or C-16(:1) omega 6c; 25.2-254%) and C-19:0 cyclo omega 8c (11.9-15.4%). The major respiratory quinone was Q-10. The genomic DNA G+C contents of PAMC 29128 and PAMC 29148(T) were 63.0 and 63.1 mol%, respectively. Their distinct phylogenetic position and some physiological characteristics support the proposal of Lichenihabitans gen. nov., with the type species Lichenihabitans psoromatis sp. nov. (type strain, PAMC 29148(T)=KCCM 43293(T)=JCM 33311(T)). Lichenihabitantaceae fam. nov. is also proposed. Y 1 |
author2 |
Hwang, Chung Yeon |
format |
Article in Journal/Newspaper |
author |
Noh, Hyun-Ju Baek, Kiwoon Hwang, Chung Yeon Shin, Seung Chul Hong, Soon Gyu Lee, Yung Mi |
author_facet |
Noh, Hyun-Ju Baek, Kiwoon Hwang, Chung Yeon Shin, Seung Chul Hong, Soon Gyu Lee, Yung Mi |
author_sort |
Noh, Hyun-Ju |
title |
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
title_short |
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
title_full |
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
title_fullStr |
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
title_full_unstemmed |
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen |
title_sort |
lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (lichenihabitantaceae fam. nov.) within the order of rhizobiales isolated from antarctic lichen |
publisher |
Society for General Microbiology |
publishDate |
2020 |
url |
https://hdl.handle.net/10371/198094 https://doi.org/10.1099/ijsem.0.003695 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
International Journal of Systematic and Evolutionary Microbiology, Vol.69 No.12, pp.3837-3842 1466-5026 https://hdl.handle.net/10371/198094 doi:10.1099/ijsem.0.003695 000500924000027 2-s2.0-85076125291 116614 |
op_doi |
https://doi.org/10.1099/ijsem.0.003695 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
69 |
container_issue |
12 |
container_start_page |
3837 |
op_container_end_page |
3842 |
_version_ |
1788062604377194496 |