Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid
To reveal the molecular mechanisms triggered by okadaic acid (OA)-exposure in the detoxification and immune system of bay scallops, we studied differentially-expressed genes (DEGs) and the transcriptomic profile in bay scallop gill tissue after 48 h exposure to 500 nM of OA using the Illumina HiSeq...
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Multidisciplinary Digital Publishing Institute (MDPI)
2019
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Online Access: | https://hdl.handle.net/10371/192524 https://doi.org/10.3390/toxins10080308 |
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ftseoulnuniv:oai:s-space.snu.ac.kr:10371/192524 2023-06-18T03:40:20+02:00 Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid Chi, Cheng Giri, Sib Sankar Jun, Jin Woo Kim, Sang Wha Kim, Hyoun Joong Kang, Jeong Woo Park, Se Chang Park, Se Chang 2019-08-02 https://hdl.handle.net/10371/192524 https://doi.org/10.3390/toxins10080308 영어 unknown Multidisciplinary Digital Publishing Institute (MDPI) Toxins, Vol.10 No.8, p. 308 2072-6651 https://hdl.handle.net/10371/192524 doi:10.3390/toxins10080308 000443617800004 2-s2.0-85051082823 80363 MUSSEL MYTILUS-GALLOPROVINCIALIS DINOFLAGELLATE PROROCENTRUM-LIMA OYSTER CRASSOSTREA-GIGAS TIME RT-PCR CHLAMYS-FARRERI RNA-SEQ PROFILING ANALYSIS REACTIVE OXYGEN PERNA-VIRIDIS DSP TOXINS harmful algal blooms okadaic acid Argopecten irradians transcriptomic response deep sequencing Article ART 2019 ftseoulnuniv https://doi.org/10.3390/toxins10080308 2023-06-02T00:47:32Z To reveal the molecular mechanisms triggered by okadaic acid (OA)-exposure in the detoxification and immune system of bay scallops, we studied differentially-expressed genes (DEGs) and the transcriptomic profile in bay scallop gill tissue after 48 h exposure to 500 nM of OA using the Illumina HiSeq 4000 deep-sequencing platform. De novo assembly of paired-end reads yielded 55,876 unigenes, of which 3204 and 2620 genes were found to be significantly up- or down-regulated, respectively. Gene ontology classification and enrichment analysis of the DEGs detected in bay scallops exposed to OA revealed four ontologies with particularly high functional enrichment, which were cellular process' (cellular component), metabolic process' (biological process), immune system process' (biological process), and catalytic process' (molecular function). The DEGs revealed that cyclic AMP-responsive element-binding proteins, acid phosphatase, toll-like receptors, nuclear erythroid 2-related factor, and the NADPH2 quinone reductase-related gene were upregulated. In contrast, the expression of some genes related to glutathione S-transferase 1, C-type lectin, complement C1q tumor necrosis factor-related protein, Superoxide dismutase 2 and fibrinogen C domain-containing protein, decreased. The outcomes of this study will be a valuable resource for the study of gene expression induced by marine toxins, and will help understanding of the molecular mechanisms underlying the scallops' response to OA exposure. Y 1 Article in Journal/Newspaper Crassostrea gigas Seoul National University: S-Space Toxins 10 8 308 |
institution |
Open Polar |
collection |
Seoul National University: S-Space |
op_collection_id |
ftseoulnuniv |
language |
unknown |
topic |
MUSSEL MYTILUS-GALLOPROVINCIALIS DINOFLAGELLATE PROROCENTRUM-LIMA OYSTER CRASSOSTREA-GIGAS TIME RT-PCR CHLAMYS-FARRERI RNA-SEQ PROFILING ANALYSIS REACTIVE OXYGEN PERNA-VIRIDIS DSP TOXINS harmful algal blooms okadaic acid Argopecten irradians transcriptomic response deep sequencing |
spellingShingle |
MUSSEL MYTILUS-GALLOPROVINCIALIS DINOFLAGELLATE PROROCENTRUM-LIMA OYSTER CRASSOSTREA-GIGAS TIME RT-PCR CHLAMYS-FARRERI RNA-SEQ PROFILING ANALYSIS REACTIVE OXYGEN PERNA-VIRIDIS DSP TOXINS harmful algal blooms okadaic acid Argopecten irradians transcriptomic response deep sequencing Chi, Cheng Giri, Sib Sankar Jun, Jin Woo Kim, Sang Wha Kim, Hyoun Joong Kang, Jeong Woo Park, Se Chang Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
topic_facet |
MUSSEL MYTILUS-GALLOPROVINCIALIS DINOFLAGELLATE PROROCENTRUM-LIMA OYSTER CRASSOSTREA-GIGAS TIME RT-PCR CHLAMYS-FARRERI RNA-SEQ PROFILING ANALYSIS REACTIVE OXYGEN PERNA-VIRIDIS DSP TOXINS harmful algal blooms okadaic acid Argopecten irradians transcriptomic response deep sequencing |
description |
To reveal the molecular mechanisms triggered by okadaic acid (OA)-exposure in the detoxification and immune system of bay scallops, we studied differentially-expressed genes (DEGs) and the transcriptomic profile in bay scallop gill tissue after 48 h exposure to 500 nM of OA using the Illumina HiSeq 4000 deep-sequencing platform. De novo assembly of paired-end reads yielded 55,876 unigenes, of which 3204 and 2620 genes were found to be significantly up- or down-regulated, respectively. Gene ontology classification and enrichment analysis of the DEGs detected in bay scallops exposed to OA revealed four ontologies with particularly high functional enrichment, which were cellular process' (cellular component), metabolic process' (biological process), immune system process' (biological process), and catalytic process' (molecular function). The DEGs revealed that cyclic AMP-responsive element-binding proteins, acid phosphatase, toll-like receptors, nuclear erythroid 2-related factor, and the NADPH2 quinone reductase-related gene were upregulated. In contrast, the expression of some genes related to glutathione S-transferase 1, C-type lectin, complement C1q tumor necrosis factor-related protein, Superoxide dismutase 2 and fibrinogen C domain-containing protein, decreased. The outcomes of this study will be a valuable resource for the study of gene expression induced by marine toxins, and will help understanding of the molecular mechanisms underlying the scallops' response to OA exposure. Y 1 |
author2 |
Park, Se Chang |
format |
Article in Journal/Newspaper |
author |
Chi, Cheng Giri, Sib Sankar Jun, Jin Woo Kim, Sang Wha Kim, Hyoun Joong Kang, Jeong Woo Park, Se Chang |
author_facet |
Chi, Cheng Giri, Sib Sankar Jun, Jin Woo Kim, Sang Wha Kim, Hyoun Joong Kang, Jeong Woo Park, Se Chang |
author_sort |
Chi, Cheng |
title |
Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
title_short |
Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
title_full |
Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
title_fullStr |
Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
title_full_unstemmed |
Detoxification- and immune-related transcriptomic analysis of gills from bay scallops (Argopecten irradians) in response to algal toxin okadaic acid |
title_sort |
detoxification- and immune-related transcriptomic analysis of gills from bay scallops (argopecten irradians) in response to algal toxin okadaic acid |
publisher |
Multidisciplinary Digital Publishing Institute (MDPI) |
publishDate |
2019 |
url |
https://hdl.handle.net/10371/192524 https://doi.org/10.3390/toxins10080308 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Toxins, Vol.10 No.8, p. 308 2072-6651 https://hdl.handle.net/10371/192524 doi:10.3390/toxins10080308 000443617800004 2-s2.0-85051082823 80363 |
op_doi |
https://doi.org/10.3390/toxins10080308 |
container_title |
Toxins |
container_volume |
10 |
container_issue |
8 |
container_start_page |
308 |
_version_ |
1769005322613030912 |