Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. cor...
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Online Access: | https://hdl.handle.net/10371/192500 https://doi.org/10.3390/pathogens9030206 |
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ftseoulnuniv:oai:s-space.snu.ac.kr:10371/192500 2023-06-18T03:40:18+02:00 Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae Kim, Hyoun Joong Jun, Jin Woo Giri, Sib Sankar Chi, Cheng Yun, Saekil Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Oh, Woo Taek Lee, Sung Bin Kim, Ji Hyung Park, Se Chang Park, Se Chang 2020-05-21 https://hdl.handle.net/10371/192500 https://doi.org/10.3390/pathogens9030206 영어 unknown MDPI AG Pathogens, Vol.9 No.3, p. 206 2076-0817 https://hdl.handle.net/10371/192500 doi:10.3390/pathogens9030206 000524306100044 2-s2.0-85081740677 101153 BACILLARY NECROSIS OSTREA-EDULIS TUBIASHII PATHOGEN SPLENDIDUS VIRULENCE METALLOPROTEASE SYSTEMS Vibrio species Pacific oyster (Crassostrea gigas) larvae genome analyses ChrI ChrII SNUTY-1 Article ART 2020 ftseoulnuniv https://doi.org/10.3390/pathogens9030206 2023-06-02T00:47:27Z Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 x 10(5) CFU/mL for 24 h caused mortality to 88.65 +/- 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to beta-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors. Y 1 Article in Journal/Newspaper Crassostrea gigas Pacific oyster Seoul National University: S-Space Pacific Pathogens 9 3 206 |
institution |
Open Polar |
collection |
Seoul National University: S-Space |
op_collection_id |
ftseoulnuniv |
language |
unknown |
topic |
BACILLARY NECROSIS OSTREA-EDULIS TUBIASHII PATHOGEN SPLENDIDUS VIRULENCE METALLOPROTEASE SYSTEMS Vibrio species Pacific oyster (Crassostrea gigas) larvae genome analyses ChrI ChrII SNUTY-1 |
spellingShingle |
BACILLARY NECROSIS OSTREA-EDULIS TUBIASHII PATHOGEN SPLENDIDUS VIRULENCE METALLOPROTEASE SYSTEMS Vibrio species Pacific oyster (Crassostrea gigas) larvae genome analyses ChrI ChrII SNUTY-1 Kim, Hyoun Joong Jun, Jin Woo Giri, Sib Sankar Chi, Cheng Yun, Saekil Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Oh, Woo Taek Lee, Sung Bin Kim, Ji Hyung Park, Se Chang Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
topic_facet |
BACILLARY NECROSIS OSTREA-EDULIS TUBIASHII PATHOGEN SPLENDIDUS VIRULENCE METALLOPROTEASE SYSTEMS Vibrio species Pacific oyster (Crassostrea gigas) larvae genome analyses ChrI ChrII SNUTY-1 |
description |
Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 x 10(5) CFU/mL for 24 h caused mortality to 88.65 +/- 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to beta-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors. Y 1 |
author2 |
Park, Se Chang |
format |
Article in Journal/Newspaper |
author |
Kim, Hyoun Joong Jun, Jin Woo Giri, Sib Sankar Chi, Cheng Yun, Saekil Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Oh, Woo Taek Lee, Sung Bin Kim, Ji Hyung Park, Se Chang |
author_facet |
Kim, Hyoun Joong Jun, Jin Woo Giri, Sib Sankar Chi, Cheng Yun, Saekil Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Oh, Woo Taek Lee, Sung Bin Kim, Ji Hyung Park, Se Chang |
author_sort |
Kim, Hyoun Joong |
title |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_short |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_full |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_fullStr |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_full_unstemmed |
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_sort |
identification and genome analysis of vibrio coralliilyticus causing mortality of pacific oyster (crassostrea gigas) larvae |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://hdl.handle.net/10371/192500 https://doi.org/10.3390/pathogens9030206 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
Pathogens, Vol.9 No.3, p. 206 2076-0817 https://hdl.handle.net/10371/192500 doi:10.3390/pathogens9030206 000524306100044 2-s2.0-85081740677 101153 |
op_doi |
https://doi.org/10.3390/pathogens9030206 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
3 |
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206 |
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1769005285916016640 |