Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island
Streptoeoccus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at...
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대한수의학회
2021
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ftseoulnuniv:oai:s-space.snu.ac.kr:10371/190796 2023-06-18T03:42:56+02:00 Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island Baeck, Gun Wook Kim, Ji Hyung Gomez, Dennis Kaw Park, Se Chang Park, Se Chang 2021-04-16 https://hdl.handle.net/10371/190796 영어 unknown 대한수의학회 Journal of Veterinary Science, Vol.7 No.1, pp.53-58 1229-845X https://hdl.handle.net/10371/190796 000238290200009 2-s2.0-33644882286 128247 ART001169925 TROUT ONCORHYNCHUS-MYKISS SCOPHTHALMUS-MAXIMUS L LACTOCOCCUS-GARVIEAE SP-NOV JUNIOR SYNONYM SP INFECTION FISH UBERIS INIAE PCR Lactococcus garvieae multiplex PCR assay Streptococcus parauberis Streptococcus sp Article ART 2021 ftseoulnuniv 2023-06-02T00:47:10Z Streptoeoccus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at a fish farm in Jeju Island, Korea. The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16 similar to 18 degrees C when samples were collected. Of the 22 fish samples collected, 3 samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test. Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCR-based method to simultaneously detect and confirm the pathogens involved in the infection. S. parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively. One fish sample was not identified because of the difference in the different biochemical and serological tests and was negative in PCR assay. In the present study, it showed that S. parauberis was the dominant species that caused streptococcosis in the cultured diseased flounder. Y 1 Article in Journal/Newspaper Scophthalmus maximus Seoul National University: S-Space Rotten ENVELOPE(-53.417,-53.417,68.867,68.867) |
institution |
Open Polar |
collection |
Seoul National University: S-Space |
op_collection_id |
ftseoulnuniv |
language |
unknown |
topic |
TROUT ONCORHYNCHUS-MYKISS SCOPHTHALMUS-MAXIMUS L LACTOCOCCUS-GARVIEAE SP-NOV JUNIOR SYNONYM SP INFECTION FISH UBERIS INIAE PCR Lactococcus garvieae multiplex PCR assay Streptococcus parauberis Streptococcus sp |
spellingShingle |
TROUT ONCORHYNCHUS-MYKISS SCOPHTHALMUS-MAXIMUS L LACTOCOCCUS-GARVIEAE SP-NOV JUNIOR SYNONYM SP INFECTION FISH UBERIS INIAE PCR Lactococcus garvieae multiplex PCR assay Streptococcus parauberis Streptococcus sp Baeck, Gun Wook Kim, Ji Hyung Gomez, Dennis Kaw Park, Se Chang Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
topic_facet |
TROUT ONCORHYNCHUS-MYKISS SCOPHTHALMUS-MAXIMUS L LACTOCOCCUS-GARVIEAE SP-NOV JUNIOR SYNONYM SP INFECTION FISH UBERIS INIAE PCR Lactococcus garvieae multiplex PCR assay Streptococcus parauberis Streptococcus sp |
description |
Streptoeoccus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at a fish farm in Jeju Island, Korea. The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16 similar to 18 degrees C when samples were collected. Of the 22 fish samples collected, 3 samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test. Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCR-based method to simultaneously detect and confirm the pathogens involved in the infection. S. parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively. One fish sample was not identified because of the difference in the different biochemical and serological tests and was negative in PCR assay. In the present study, it showed that S. parauberis was the dominant species that caused streptococcosis in the cultured diseased flounder. Y 1 |
author2 |
Park, Se Chang |
format |
Article in Journal/Newspaper |
author |
Baeck, Gun Wook Kim, Ji Hyung Gomez, Dennis Kaw Park, Se Chang |
author_facet |
Baeck, Gun Wook Kim, Ji Hyung Gomez, Dennis Kaw Park, Se Chang |
author_sort |
Baeck, Gun Wook |
title |
Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
title_short |
Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
title_full |
Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
title_fullStr |
Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
title_full_unstemmed |
Isolation and characterization of Streptococcus sp from diseased flounder (Paralichthys olivaceus) in Jeju Island |
title_sort |
isolation and characterization of streptococcus sp from diseased flounder (paralichthys olivaceus) in jeju island |
publisher |
대한수의학회 |
publishDate |
2021 |
url |
https://hdl.handle.net/10371/190796 |
long_lat |
ENVELOPE(-53.417,-53.417,68.867,68.867) |
geographic |
Rotten |
geographic_facet |
Rotten |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_relation |
Journal of Veterinary Science, Vol.7 No.1, pp.53-58 1229-845X https://hdl.handle.net/10371/190796 000238290200009 2-s2.0-33644882286 128247 ART001169925 |
_version_ |
1769009130489511936 |