Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality
Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the sprea...
Published in: | Pathogens |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | http://hdl.handle.net/11388/236221 https://doi.org/10.3390/pathogens9060492 |
id |
ftsassariuniiris:oai:iris.uniss.it:11388/236221 |
---|---|
record_format |
openpolar |
spelling |
ftsassariuniiris:oai:iris.uniss.it:11388/236221 2024-04-14T08:18:01+00:00 Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality Mandas D. Salati F. Polinas M. Sanna M. A. Zobba R. Burrai G. P. Alberti A. Antuofermo E. Mandas, D. Salati, F. Polinas, M. Sanna, M. A. Zobba, R. Burrai, G. P. Alberti, A. Antuofermo, E. 2020 http://hdl.handle.net/11388/236221 https://doi.org/10.3390/pathogens9060492 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000551113300001 volume:9 issue:6 firstpage:1 lastpage:12 numberofpages:12 journal:PATHOGENS http://hdl.handle.net/11388/236221 doi:10.3390/pathogens9060492 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088229799 Aquaculture Bacteria Crassostrea giga Histology Mass mortality Real time PCR Sardinia info:eu-repo/semantics/article 2020 ftsassariuniiris https://doi.org/10.3390/pathogens9060492 2024-03-15T04:05:43Z Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the spread of pathogens around the world. Combined with environmental changes and intensive production systems this has contributed to the occurrence of mass mortality episodes, thus posing a threat to the production of different species, including the Pacific oyster Crassotrea gigas. In the San Teodoro lagoon, one of the most devoted lagoons to extensive Pacific oyster aquaculture in Sardinia, a mortality outbreak was observed with an estimated 80% final loss of animal production. A study combining cultural, biomolecular and histopathological methods was conducted: (1) to investigate the presence of different Vibrio species and OsHV-1 in selected oyster tissues (digestive gland, gills, and mantle); (2) to quantify Vibrio aestuarianus and to evaluate the severity of hemocyte infiltration in infected tissues; (3) to produce post-amplification data and evaluating ToxR gene as a target for phylogenetic analyses. Results provide new insights into V. aestuarianus infection related to oyster mortality outbreaks and pave the way to the development of tools for oyster management. Article in Journal/Newspaper Pacific oyster CINECA IRIS Universitá Degli Studi di Sassari Pacific Pathogens 9 6 492 |
institution |
Open Polar |
collection |
CINECA IRIS Universitá Degli Studi di Sassari |
op_collection_id |
ftsassariuniiris |
language |
English |
topic |
Aquaculture Bacteria Crassostrea giga Histology Mass mortality Real time PCR Sardinia |
spellingShingle |
Aquaculture Bacteria Crassostrea giga Histology Mass mortality Real time PCR Sardinia Mandas D. Salati F. Polinas M. Sanna M. A. Zobba R. Burrai G. P. Alberti A. Antuofermo E. Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
topic_facet |
Aquaculture Bacteria Crassostrea giga Histology Mass mortality Real time PCR Sardinia |
description |
Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the spread of pathogens around the world. Combined with environmental changes and intensive production systems this has contributed to the occurrence of mass mortality episodes, thus posing a threat to the production of different species, including the Pacific oyster Crassotrea gigas. In the San Teodoro lagoon, one of the most devoted lagoons to extensive Pacific oyster aquaculture in Sardinia, a mortality outbreak was observed with an estimated 80% final loss of animal production. A study combining cultural, biomolecular and histopathological methods was conducted: (1) to investigate the presence of different Vibrio species and OsHV-1 in selected oyster tissues (digestive gland, gills, and mantle); (2) to quantify Vibrio aestuarianus and to evaluate the severity of hemocyte infiltration in infected tissues; (3) to produce post-amplification data and evaluating ToxR gene as a target for phylogenetic analyses. Results provide new insights into V. aestuarianus infection related to oyster mortality outbreaks and pave the way to the development of tools for oyster management. |
author2 |
Mandas, D. Salati, F. Polinas, M. Sanna, M. A. Zobba, R. Burrai, G. P. Alberti, A. Antuofermo, E. |
format |
Article in Journal/Newspaper |
author |
Mandas D. Salati F. Polinas M. Sanna M. A. Zobba R. Burrai G. P. Alberti A. Antuofermo E. |
author_facet |
Mandas D. Salati F. Polinas M. Sanna M. A. Zobba R. Burrai G. P. Alberti A. Antuofermo E. |
author_sort |
Mandas D. |
title |
Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
title_short |
Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
title_full |
Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
title_fullStr |
Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
title_full_unstemmed |
Histopathological and molecular study of pacific oyster tissues provides insights into V. Aestuarianus infection related to oyster mortality |
title_sort |
histopathological and molecular study of pacific oyster tissues provides insights into v. aestuarianus infection related to oyster mortality |
publishDate |
2020 |
url |
http://hdl.handle.net/11388/236221 https://doi.org/10.3390/pathogens9060492 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000551113300001 volume:9 issue:6 firstpage:1 lastpage:12 numberofpages:12 journal:PATHOGENS http://hdl.handle.net/11388/236221 doi:10.3390/pathogens9060492 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088229799 |
op_doi |
https://doi.org/10.3390/pathogens9060492 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
6 |
container_start_page |
492 |
_version_ |
1796317412502536192 |